畜牧兽医学报2013，Vol.44Issue(5)：673-680,8.DOI:10.11843/j.issn.0366-6964.2013.05.002
湖羊催乳素受体基因外显子10多态性及与其母性行为性状的关联分析
Analysis on Genetic Polymorphism of Exon 10 of PRLR Gene and Its Association with Distribution Type of Its Maternal Behavior in Hu Sheep
摘要
Abstract
The aim of this study was to study the genetic diversity of PRLR gene in Hu sheep to discuss its effect on Hu sheep maternal behaviors and provide the genetics analysis method for evaluating sheep maternal behaviors.81 parturition ewes were used to detect single nucleotide polymorphism of PRLR gene exon10 by using PCR-SSCP,in which 63 individuals were observed for maternal behaviors.The methods of normal distribution test,cluster analysis and nonparametric test were used to reveal the Hu sheep maternal behavior distribution rules and the difference of Hu sheep maternal behavior with various genotypes.The result showed that only the products amplified by primers P3 displayed polymorphisms.The genotypes were AA,AB and BB,and each genotype fitted Hardy-Weinberg equilibrium.The statistical data of licking,nursing,treading and refusing to feed lamb didn't fit normal distribution.The results of nonparametric test with multiple independent samples showed that the difference of observations for licking,nursing and trampling of individuals with AA,AB and BB genotypes was extremely significant (P＜0.01).No obvious difference was found in individuals with three genotypes for the behaviour of refusing to feed young sheep (P＞0.05).As the result showed,the partial sequences of PRLR gene exon10 had polymorphisms and there was correlationship between PRLR gene and maternal behaviours.According to the preliminary inference,PRLR gene could be used as candidate gene affecting the Hu sheep maternal behavior.关键词
湖羊/催乳素受体基因/多态性/母性行为Key words
Hu sheep/PRLR gene/polymorphisms/maternal behavior分类
农业科技引用本文复制引用
孙伟,张向楠,左其生,倪蓉,王兰萍,张有法,陈玲,吴文忠,周洪..湖羊催乳素受体基因外显子10多态性及与其母性行为性状的关联分析[J].畜牧兽医学报,2013,44(5):673-680,8.基金项目
江苏省农业科技支撑计划项目(BE2012331) （BE2012331）
科技部家养动物种质资源平台 （）
江苏高校优势学科建设工程资助项目 （）
江苏省研究生培养创新工程项目 （）
国家自然科学基金(31101747) （31101747）
江苏省六大人才高峰项目 （）
江苏省工程技术研究中心项目(BM2012308) （BM2012308）
