畜牧兽医学报2013，Vol.44Issue(5)：681-689,9.DOI:10.11843/j.issn.0366-6964.2013.05.003
不同羊毛纤维直径细毛羊皮肤组织差异表达基因研究
Genome Array on Differentially Expressed Genes of Skin Tissue in Fine Wool Sheep with Different Fiber Diameter
摘要
Abstract
The study aimed to screen the differentially expressed genes of skin tissue in fine wool sheep with different fiber diameter.Affymetrix GeneChip (R) Bovine Genome Array and Agilent Sheep Oligo Microarray were used to screen differentially expressed genes of skin tissue in fine wool sheep with different fiber diameter.Significance Analysis of Microarray (SAM) methods was used to identify the differentially expressed genes.GO (Gene Ontology) and the pathway analyses were conducted on differentially expressed genes using a free web-based Molecular Annotation System 3.0 (MAS 3.0).A total of 267 genes were identified to be differentially expressed genes from 2 different Microarray,106 were up-regulated expression genes and 161 were down-regulated expression genes.GO and Kegg analysis revealed that the expression levels of the most differentially expressed genes were related to cell proliferation,differentiation,apoptosis,protein biosynthesis,Wnt signaling pathway and Keratin constitution etc.Subsequently,Realtime PCR was performed to validate seven differentially expressed genes which were screened out by the microarray approach and sufficient consistency was observed by the two methods.The results suggest that the gene expression profiles of fine wool sheep with different fiber diameter are variant,their different expression may alter the formation and differentiation of skin follicle and wool fiber,and some important genes identified may be useful in further study on molecular markers of fiber diameter in fine wool sheep.关键词
细毛羊/纤维直径/基因表达/基因芯片Key words
fine wool sheep/fiber diameter/gene expression/microarray分类
农业科技引用本文复制引用
狄江,拉扎提·艾尼瓦尔,徐新明,张艳花,田可川,田月珍,吴伟伟,于丽娟,哈尼克孜·吐拉甫..不同羊毛纤维直径细毛羊皮肤组织差异表达基因研究[J].畜牧兽医学报,2013,44(5):681-689,9.基金项目
国家科技支撑项目(2011BAD28B05) （2011BAD28B05）
国家绒毛用羊产业技术体系(CARS-40) （CARS-40）
新疆重大专项(200731132-7) （200731132-7）
