畜牧兽医学报2013，Vol.44Issue(5)：690-696,7.DOI:10.11843/j.issn.0366-6964.2013.05.004
澳系进口荷斯坦牛IL8基因遗传多态性与体细胞评分及泌乳性状的关联分析
Genetic Polymorphism of IL8 Gene and Its Association with Milk Traits and SCS in Holstein Imported from Australia
摘要
Abstract
The effect of two mutations in Interleukin-8 (IL-8) gene for 588 Holstein cows from 72 bull families of Haifeng dairy cattle farm imported from Australia were investigated on 7 milking performances:tested day milk yield,milk fat percentage,milk protein percentage,305 d corrected milk yield,305 d milk fat yield,305 d milk protein yield and somatic cell score (SCS).The aim was to explore molecular marker in the breeding of dairy cattle.The polymorphism of IL8 gene was detected by polymerase Chain reaction-single strand conformation polymorphism (PCRSSCP) technique.The association between polymorphic sites of IL8 gene and milking performances,SCS was analyzed by the least square mean model.The results showed that a mutation from G to A was identified in the 5'upstream of-180(G/A),the genotypic frequencies of GG,GA and AA were 0.226,0.476 and 0.298,respectively.The gene frequencies of G and A were 0.464 and 0.536,respectively.No significant effects of mutation were observed on milking performances and SCS except on 305 d milk fat yield.The 305 d milk fat yield for GG genotype was higher than GA (P＜0.05).A mutation from A to G was identified in 2 789 of IL8,and the genotypic frequencies of GG,GA and AA were 0.297,0.506 and 0.197,respectively.The gene frequencies of G and A were 0.550 and 0.450.This mutation showed significant effects on tested day milk fat percentage,milk protein percentage,305 d corrected milk yield and SCS.The AA genotype had greatly significant higher milk fat percentage than GG and GA genotypes (P＜0.01).Milk protein percentage for AA was greatly significant higher than that GA (P＜0.01).GG genotype had greatly significant higher SCS than GA and AA (P＜0.01).305 d corrected milk yield for GA was significant higher than AA (P＜0.05).The polymorphisms of IL8 gene had a greatly genetic effects on milking traits and mastitis resistance,so this gene could be a useful genetic marker for Holstein cows imported from Australia.关键词
澳系进口荷斯坦牛/IL8基因/泌乳性状/遗传多态性/SCSKey words
Holstein cows imported from Australia/IL8 gene/milking traits/genetic polymorphism/SCS分类
农业科技引用本文复制引用
张美荣,陈丹,廖想想,毛永江,刘坤,陈亮,王杏龙,杨章平,杨利国..澳系进口荷斯坦牛IL8基因遗传多态性与体细胞评分及泌乳性状的关联分析[J].畜牧兽医学报,2013,44(5):690-696,7.基金项目
江苏省苏北专项(BN2011016) （BN2011016）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
中国博士后基金(20110491169) （20110491169）
扬州大学科技创新培育基金(2011CXJ059) （2011CXJ059）
