畜牧兽医学报2013，Vol.44Issue(5)：754-759,6.DOI:10.11843/j.issn.0366-6964.2013.05.012
1株髓细胞瘤型J亚群禽白血病病毒感染性克隆的构建与病毒拯救
Construction and Virus Rescue of Infectious Molecular Clones of Subgroup J Avian Myeloid Leukosis Virus
摘要
Abstract
The objective of this study was to construct the infectious molecular clone with molecular marker of subgroup J avian myeloid leukosis virus (ALV-J) strain SCGS-1.A full-length infectious clone of ALV-J (pUC-SCGS) was constructed by cloning and combining of three fragments using PCR method from SCGS-1.Sal Ⅰ site was introduced on 4684nt of SCGS-1 by overlapping PCR to form another infectious clone and named pUC-△SCGS.The two plasmids,pUCSCGS and pUC-△SCGS,were transfected into CEF,and the rescued viruses were detected by PCR,avian leukosis virus antigen test kit and indirect immunofluorescence assay (IFA).Digestion and sequence analysis revealed that the infectious clone pUC-SCGS and pUC-△SCGS were constructed correctly.PCR,ELISA test and IFA results showed that the 3rd and 4th generation of rescued virus were positive,while the controlled CEF were negative.Rescued virus and the virus with molecular marker of subgroup J avian myeloid leukosis virus were successfully constructed,named rSCGS-1 and r△SCGS-1.关键词
髓细胞瘤型/J亚群禽白血病病毒/感染性克隆/分子标记/病毒拯救Key words
myeloid leukosis/subgroup J avian leukosis virus/infectious molecular clone/molecular marker/virus rescue分类
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林艳,夏静,邹年莉,郭明萍,王富妍,赵扬,文心田,曹三杰,黄勇..1株髓细胞瘤型J亚群禽白血病病毒感染性克隆的构建与病毒拯救[J].畜牧兽医学报,2013,44(5):754-759,6.基金项目
教育部《长江学者和创新团队发展计划》创新团队项目(IRTO848) （IRTO848）
