畜牧兽医学报2013，Vol.44Issue(5)：772-777,6.DOI:10.11843/j.issn.0366-6964.2013.05.015
猪肺炎支原体黏附猪肾细胞模型建立及体外黏附特性研究
Establishment of Mycoplasma hyopneumoniae Adhesion on PK15 Model and the Adhesion Ability in vitro
摘要
Abstract
This experiment was conducted to develop the infection model of Mycoplasma hyopneumoniae in vitro and study the adhesion characteristics by indirect immunofluorescence method in different cell lines.Several Mhp strains which include field strains (XLW-1,XLW-2,XLW-3,WX),international standard strain 232 and the attenuated strain (168) were selected,and infected Pig Kidney15 (PK15).The results showed that all the Mhp whole bacteria strains could adhere to PK15 cell lines with different adhesion efficiency.And the order of adhesion ability of Mhp was field strain XLW-1,WX,the attenuated strain (168),international standard strain 232,field strain XLW-2,XLW-3.The field strain XLW-1 had the best adhesion ability,so it was selected as test strain to study the adhesion ability to other cell lines,such as the original generated cells of pig tracheal epithelial,porcine alveolar epithelial cells (SJPL),porcine alveolar macrophages (3D4/31) and rhesus monkey kidney cells (Marc145).XLW-1 strains can adhere to different cell lines in vitro,but the different adhesion capacity also showed on the different cell lines.XLW-1 strain has good adhesion ability to pig tracheal epithelial cells in primary separation,the SJPL cell lines and 3D4/31 cell lines,while very weak ability to the non-swine cell lines,Marc145 cell line.In conclusion,Mycoplasma hyopneumoniae adhesion model was developed on different cell lines,and different Mhp strains showed different adhesion ability on the same cell lines,as well as the same strain showed the different adhesion on different cell lines.关键词
猪肺炎支原体/体外感染模型/黏附特性Key words
Mycoplasma hyopneumoniae/in vitro infection model/adhesion characteristics分类
农业科技引用本文复制引用
张旭,冯志新,武昱孜,车巧林,刘茂军,熊祺琰,华利忠,李彬,邵国青..猪肺炎支原体黏附猪肾细胞模型建立及体外黏附特性研究[J].畜牧兽医学报,2013,44(5):772-777,6.基金项目
国家自然科学基金(31001080 （）
31001066) （）
中国博士后基金(2012M510175) （2012M510175）
