中国农业大学学报2013，Vol.18Issue(4)：1-6,6.DOI:10.11841/j.issn.1007-4333.2013.04.01
小麦抗叶锈病基因LrAlt的比较基因组学分析
Comparative genomics analysis of the wheat leaf rust resistance gene LrAlt
摘要
Abstract
Wheat leaf rust resistance gene LrAlt was identified in spelt wheat (Triticum spelta L.) accession Altgold and located on the distal part of the short arm of wheat chromosome 2A.Comparative genomics analysis showed that the genomic region containing LrAlt on 2AS was orthologous to Brachypodium (Brachypodium distachyon) chromosome 5 and rice (Oryza sativa L.) chromosome 4.Based on wheat-Brachypodium-rice genomic colinearity,EST-STS markers,BE498683,BE471132.1,BG605273,and CD454629 were linked to LrAlt.These four EST-STS markers cosegregating with Xbarc212 were proximal to LrAlt with a genetic distance of 1.9 cM.According to Graingenes 2.0,additional SSRs neighbouring the locus LrAlt were tested.Xbarc124 and Xgwm614 were confirmed to be linked to LrAlt,both of which were congregated with Xbarc212.By comparative genomics analysis and screening of SSRs,9markers linked to LrAlt were established,which provide invaluable information for fine mapping,molecular assisted selection,and gene pyramiding of LrAlt.关键词
小麦叶锈病/LrAlt/比较基因组学/EST-STS/SSRKey words
wheat leaf rust/ LrAlt/ comparative genomics/ EST-STS/ SSR分类
农业科技引用本文复制引用
唐华山,彭福祥,王艳洁,沈红霞,伊黛舒,李保云,彭惠茹,刘志勇,解超杰..小麦抗叶锈病基因LrAlt的比较基因组学分析[J].中国农业大学学报,2013,18(4):1-6,6.基金项目
国家自然科学基金资助项目(31271708) （31271708）
国家"973"计划项目(2009CB118300) （2009CB118300）
