中国农业大学学报2013，Vol.18Issue(4)：141-146,6.DOI:10.11841/j.issn.1007-4333.2013.04.22
奶牛酮病与胰岛素抵抗关系探讨
Study on the relationship of ketosis and insulin resistance in dairy cows
摘要
Abstract
To study the relationship between ketosis and insulin resistance during early lactation,16 ketosis (T) and 24 healthy control (C) cows were chosen from a intensive dairy farm.Blood samples from all cows were collected at 14 days postpartum during morning fasting.Differences in energy balance,liver function,oxidative stress,insulin sensitivity and glucose tolerance were analyzed between T and C.Results showed that,cows with keosis were in condition of negative energy balance.The level of plasma AST and DBIL raised significantly (P＜0.01) in T group compared to C group,and plasma TBIL also increased significantly (P＜0.05),but plasma CHE and TP decreased significantly (P＜0.01 or ＜0.05),and there is no significantly change in plasma ALT,IBIL,ALB,and GLO,suggesting that cows with ketosis suffered from certain degree of liver function abnormality.In addition,level of plasma malondialdehyde (MDA)and superoxide dismutase (SOD) was significantly higher in T group than in C group (P＜0.05),implied that cows affected ketosis experienced oxidative stress.Furthermore,plasma revised quantitative insulin sensitivity test (RQUICKI) was lower significantly in T group than in C group (P＜0.05),indicated that cows with ketosis had low insulin sensitivity.Concentration of plasma GIc in T group were higher than in C group during glucose tolerance test (P＜0.05),and plasma GIc concentration was still 4.24 mmol/L at 120 min after intravenous injection of 50％ dextrose,which had a significant differences between two groups (P =0.006),showing that cows affected ketosis had insulin resistance.It is concluded that there is a closed relationship between ketosis and insulin resistance,which may be related to liver function and oxidative stress because both can cause insulin resistance.关键词
奶牛/酮病/能量负平衡/肝功能状况/氧化应激/胰岛素抵抗Key words
dairy cows/ ketosis/ negative energy balance/ liver function/ oxidative stress/ insulin resistance分类
农业科技引用本文复制引用
孙照磊,王朋贤,舒适,刘健男,王博,姚远,夏成,张洪友,王哲..奶牛酮病与胰岛素抵抗关系探讨[J].中国农业大学学报,2013,18(4):141-146,6.基金项目
"十二五"农村领域国家科技课题(2012BAD12B05-2,2012/BAD12B03-2) （2012BAD12B05-2,2012/BAD12B03-2）
国家自然科学基金面上项目(31072181) （31072181）
黑龙江省农垦总局项目(HNK11A-08-01-09) （HNK11A-08-01-09）
