中国农业大学学报2013，Vol.18Issue(4)：155-161,7.DOI:10.11841/j.issn.1007-4333.2013.04.24
输卵管上皮细胞共培养促进小鼠早期胚胎发育机理的探索
To explore the development promoting mechanism of oviduct epithelial cells co-culture on the early embryos in mouse
摘要
Abstract
The objective of this study was to investigate the effect of oviduct epithelial cells on the development and quality of early embryos,as well as to study the impact of embryos on the activity and gene transcriptions of co-cultured oviduct epithelial cells,and explore the mechanisms on how oviduct epithelial cells improving the development of early embryo in mouse.Mouse oviduct epithelial cell lines were purified and used to co-culture with zygotes,H2O2 content in embryos was measured by DCHFDA,epithelial cell activity was measured by MTT,RT-PCR was applied to test the transcription of CAT and GPX gene.Results are as follows:1) co-culture can significantly improve the early 2-cell and 4cell rates (0.951 3％ VS 0.903 3％,0.857 6％ VS 0.700 5％,P＜0.05) as well as blastocyst rate (0.537 9％ VS 0.573 3％,P＞0.05) ；2) it can decrease the H2O2 content in early 2-cell embryos (0.047 9 VS 0.068 2,P＜0.05) ；3) rates of type Ⅰ and Ⅱ arrested embryos tend to decrease (0.158 3％ VS 0.192 6％,0.213 3％ VS 0.433 9％,P＞0.05),rate of type Ⅲ arrested embryos was increased(0.621 6％ VS 0.373 3％,P＜0.05) ；4) cellular activity had improving trend when embryos developed to early 2-cell phase(0.216 7 VS 0.195 7,P＞0.05) ；5) the transcription of CAT and GPX were slightly increased(1.202 0 VS 0.984 9,1.310 1 VS 0.998 3,P＞0.05)though not significant.Those results indicated that oviduct epithelial cell co-culture can improve the development of embryos by increasing anti-oxidative ability of themselves,then decreasing ROS content in embryos by cell-cell contact,therefore it can improve embryo quality and promote embryo development.关键词
输卵管上皮细胞/共培养/早期胚胎/阻滞/活性氧/小鼠Key words
oviduct epithelial cells/ co-culture/ early embryos/ arrest/ ROS/ mouse分类
农业科技引用本文复制引用
庄利利,胡德宝,蔡丽娟,张也,李钟淑,方南洙..输卵管上皮细胞共培养促进小鼠早期胚胎发育机理的探索[J].中国农业大学学报,2013,18(4):155-161,7.基金项目
延边大学211工程第三期重点学科建设项目(2009-002) （2009-002）
动物高效快繁及胚胎工程高新技术研究 （）
