中国农业大学学报2013，Vol.18Issue(4)：168-177,10.DOI:10.11841/j.issn.1007-4333.2013.04.26
产细菌素乳酸菌的筛选及细菌素相关基因的分析
Screening of bacteriocin producing lactic acid bacteria and the analysis of bacteriocin related genes
摘要
Abstract
In order to obtain a bacteriocin producing strain,lactic acid bacteria were separated and screened with dissolve calcium circle and oxford cup double-plate method from premium fermentation meat.Morphological,physiological biochemistry method,16S rDNA and specific gene PCR amplification were used to identify the strain to species,and the bacteriocin related gene was analyzed to determine bacteriocin types.The results showed that,after eliminating organic acid,hydrogen peroxide and other interference factors,L-ZS9 still had antibacterial activity among the 92 strains and its antibacterial substances were sensitive to protease (acid protease,protease K,trypsin,pepsin,neutral protease),while insensitive to alpha amylase.Therefore,L-ZS9 was recognized as bacteriocin producing bacteria and identified as Lactobacillus paraplantarum.Further bacteriocin related gene PCR amplification showed that L-ZS9 contained Ⅱ b plantaricins EF,JK,Ⅱ c plantaricin A and plantaricin N encoding gene.The mature peptide sequences of plnF,plnK were identical with Lactobacillus plantarum C11 (X94434) and only one amino acid mutation occurred in pinE,plnJ mature peptide sequences.Therefore,the Lactobacillus paraplantarum L-ZS9 was a nature multibacteriocinogenic strain.关键词
乳酸菌/筛选/细菌素/类植物乳杆菌Key words
lactic acid bacteria/ screening/ bacteriocin/ Lactobacillus paraplantarum分类
轻工纺织引用本文复制引用
张旭,赵斌,张香美,李平兰..产细菌素乳酸菌的筛选及细菌素相关基因的分析[J].中国农业大学学报,2013,18(4):168-177,10.基金项目
国家自然科学基金项目(31071591) （31071591）
产业技术体系北京市创新团队项目(SCGWZJ 20121105-2) （SCGWZJ 20121105-2）
