中国农业科学2013，Vol.46Issue(5)：1005-1013,9.DOI:10.3864/j.issn.0578-1752.2013.05.016
24-表油菜素内酯对低温胁迫下葡萄幼苗抗氧化系统及渗透调节物质的影响
Effects of 24-Epibrassinolide on the Antioxidant System and Osmotic Adjustment Substance in Grape Seedlings (V.vinifera L.) Under Chilling Stress
摘要
Abstract
[Objective] This study was conducted to determine the influence of the exogenous EBR (24-Epibrassinolide) on the antioxidant system and osmotic adjustment substance of the grape seedlings under chilling stress.[Method] With table grape cultivar ‘Zicuiwuhe’ (Vitis vinifera L.) as experimental material and were grown in nutrition bags,0.1 mg.L-1 EBR was sprayed on grape seedling leaves,the effects of the exogenous EBR on four antioxidant enzyme activities (CAT,SOD,POD and APX),superoxide anion (O2-),malondialdehyde (MDA) content,free proline,soluble sugar and the soluble protein content of grape seedling under low temperature of 4℃ and 0℃ were compared with water (control).[Result] Under the condition of two low temperature stress,grape seedling leaves,treated with exogenous EBR,the activities of antioxidative enzymes such as ascorbate peroxidase (APX),catalase (CAT),peroxidase (POD) and superoxide dismutase (SOD) increased compared with the control,and had significant differences between the EBR treatment and control.The EBR treatment also greatly enhanced the contents of ascorbic acid (AsA)and glutathione (GSH) during chilling stress,compared to the control,the average content of GSH and AsA increased by 5.4％ and 7.8％,8.8％ and 13.0％ under 4℃ and 0℃ conditions,respectively.However the content of O2-and MDA decreased by 28.5％ and 17.3％,6.8％ and 7.5％,respectively.The leaves of grape seedlings which were treated with EBR improved the contents of flee proline,soluble sugar and soluble protein,as a result,the average content of them increased by 8.4％ and 59.1％,3.5％ and 5.2％,18.6％ and 25.0％ under 4℃ and 0℃,respectively.[Conclusion] Exogenous EBR reduced the accumulation of reactive oxygen species (ROS) and the content of MDA,and could play positive roles in the alleviation of oxidative damage caused by ROS overproduction through enhancing antioxidant defense system,meanwhile enhanced the grape seedlings osmotic adjustment substance,resulting in improving the tolerance of grape seedlings to chilling stress.关键词
葡萄幼苗/低温胁迫/抗氧化系统/渗透调节物质Key words
grape seedling/ chilling stress/ antioxidant system/ osmotic adjustment substance引用本文复制引用
惠竹梅,王智真,胡勇,邓敏敏,张振文..24-表油菜素内酯对低温胁迫下葡萄幼苗抗氧化系统及渗透调节物质的影响[J].中国农业科学,2013,46(5):1005-1013,9.基金项目
现代农业产业技术体系建设专项(CARS-30-zp-9)、陕西省自然科学基金项目(2011JM3004)、西北农林科技大学基本科研业务费专项(QN2009059) （CARS-30-zp-9）
