中国农业科学2013，Vol.46Issue(5)：1036-1043,8.DOI:10.3864/j.issn.0578-1752.2013.05.019
牦牛和犏牛Dmrt7基因序列分析及其在睾丸组织中的表达水平
Sequence Analysis and Study on the Expression Level of Dmrt7 Gene in Yak and Cattle-Yak Testis
摘要
Abstract
[Objective] The aim of the study is to investigate the coding region sequences,their structures and the expression levels of Dmrt7 gene in yak and cattle-yak testis,for offering references for the revealing of the molecular mechanism of infertility of cattle-yak.[Method] A coding region sequence of yak and cattle-yak Dmrt7 was cloned by molecular cloning technique in this study.Some characters of the Dmrt7 gene and encoded protein sequences were predicted and analyzed by the methods of bioinformatics in the following aspects as the functional sites and secondary structure.The expression levels of Dmrt7 mRNA in some organs of yak were detected by semi-quantitative PCR.Real-time PCR was employed to examine the expression levels of Dmrt7 mRNA in yak and cattle-yak testis.Western blotting was applied to detect the expression levels of Dmrt7 protein in yak and cattle-yak testis.[Result] The coding region sequences of Dmrt7 gene in yak and cattle-yak were consistent,contains a complete ORF (1 113 bp) which encoding 370 amino acids.The protein encoded by yak and cattle-yak Dmrt7 contains a DM domain.The secondary structures were mainly composed of Helix,Coli and Extended strand.Dmrt7 mRNA expression in the testis was detected,but not in any other tissue tested.And the difference of expression level of Dmrt7 mRNA and protein in testis between yak and cattle-yak was extremely remarkable (P＜ 0.01).[Conclusion] The expression levels of Dmrt7 mRNA and protein in yak testis were significantly higher than cattle-yak testis,and the expression levels of Dmrt7 mRNA and protein were consistent.The results indicate that the expression level of Dmrt7 protein in cattle-yak may be too low to block the meiosis process.关键词
牦牛/犏牛/睾丸组织/Dmrt7基因/克隆/生物信息学分析/表达水平Key words
yak/ cattle-yak/ testis/ Dmrt7/ cloning/ bioinformaticanalysis/ expression level引用本文复制引用
金帅,郭宪,包鹏甲,梁春年,吴晓云,刘建,阎萍..牦牛和犏牛Dmrt7基因序列分析及其在睾丸组织中的表达水平[J].中国农业科学,2013,46(5):1036-1043,8.基金项目
现代农业(肉牛牦牛)产业技术体系专项(CARS-38)、甘肃省科技重大专项(1102NKDA027)、国家公益性行业(农业)科研专项(201003061) （肉牛牦牛）
