中国农业科学2013，Vol.46Issue(6)：1237-1246,10.DOI:10.3864/j.issn.0578-1752.2013.06.018
FSH对体外培养仔猪睾丸支持细胞cyclin D1 mRNA和cyclin E1 mRNA表达的影响
Effect of FSH on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell
摘要
Abstract
[Objective] The objective of the study is to identify whether or how FSH regulates the mRNA expression of cyclin D1 and cyclin E1 in the cultured immature boar Sertoli cell.[Method] In the study,the cultured immature boar Sertoli cells were used as materials,and real-time PCR was used to detect the mRNA expression ofcyclin D1 and cyclin El.[Result] Different doses of FSH (0-100 ng·mL-1) increased the mRNA expression of cyclin D1 and cyclin E1 (P＜0.05),which were peaking at 50 ng·mL-1 (P＜0.05).In addition,FSH (50 ng·mL-1) increased the mRNA expression of cyclin D1 and cyclin E1 in a time-dependent manner (P＜0.05) and peaked at 30 min (P＜0.05).Different doses of Forskolin (0-20 μmol·L-1) increased the mRNA expression of cyclin D1 and cyclin E1 (P＜0.05),but lower compared with FSH alone.Rp-cAMP (0-40 μmol·L-1),H-89 (0-30 μmol·L-1) and Verapamil (0-100 μg·mL-1) inhibited FSH induced the mRNA expression of cyclin D1 and cyclin E1 in a dose-dependent manner (P＜0.05),and the combination of Rp-cAMP and Verapamil had more inhibition on FSH activity than individual effect (P＜0.05),but lower than addition of Rp-cAMP and Verapamil.Furthermore,different doses of U0126 (0-10 μmol·L-1) reduced F SH induced the mRNA expression of cyclin D1 and cyclin E1 (P＜0.05),while Rp-cAMP,H-89,Verapamil or U0126 alone had no effect on the mRNA expression of cyclin D1 and cyclin E1 as compared to the control (P＞0.05).[Conclusion] FSH regulates the mRNA expression of cyclin D1 and cyclin E1 in a dose-and time-dependent manner,while cAMP-PKA,Ca2+ and extracellular signal-regulated kinase (ERK1/2) is involved in the regulation of F SH on the mRNA expression of cyclin D1 and cyclin E1.关键词
促卵泡素/睾丸支持细胞/细胞周期蛋白D1/细胞周期蛋白E1Key words
follicle-stimulating hormone/ sertoli cell/ cyclin D1/ cyclin E1引用本文复制引用
张姣姣,王怡,张会琼,孙思,孙燕,王鲜忠,张家骅..FSH对体外培养仔猪睾丸支持细胞cyclin D1 mRNA和cyclin E1 mRNA表达的影响[J].中国农业科学,2013,46(6):1237-1246,10.基金项目
国家自然科学基金项目(31072183、31101872)、西南大学研究生科技创新基金项目(ky2011005) （31072183、31101872）
