中国农业科学2013，Vol.46Issue(6)：1256-1262,7.DOI:10.3864/j.issn.0578-1752.2013.06.020
莱克多巴胺表面分子印迹材料的制备、表征及吸附性能
Preparation and Characterization of Ractopamine-Imprinted Material Using Surface-Molecular Imprinting Method and Its Adsorption Characteristics
摘要
Abstract
[Objective] The objective of this experiment is to prepare a molecule-imprinted material on surface of silica gel for ractopamine.[Method] Polymethylacrylic acid (PMAA) was grafted onto the surface of silica gel particles via the coupling effect of γ-methacryloxypropyl trimethoxy silane,and the grafting particle PMAA/SiO2 was prepared.Then,the molecular imprinting towards the grafted PMAA was performed with ractopamine as template molecule and ethylene glycol diglycidylether (EGDE) as crosslinking agent by using the surface-molecular imprinting technique established,and the ractopamine molecule-imprinted material MIP-PMAA/SiO2 was prepared.The morphology of MIP-PMAA/SiO2 was observed by SEM,and the chemical structure of MIP-PMAA/SiO2 was characterized by FT-IR.Static method was adopted to study the binding properties and molecule recognition characters of MIP-PMAA/SiO2 for ractopamine.[Result] The MIP-PMAA/SiO2 on the adsorption ability of ractopamine was significantly better than clenbuterol and salbutamol.The adsorption capacity of the MIP-PMAA/SiO2 was 11.08 mg·g-1,the specificity binding capacity was 9.93 mg.g-1,and the imprinted factor of the polymer was 9.63.The adsorption reaction reached equilibrium after 10 min,and the desorption ration of ractopamine reached 92.3％ in 5 min.[Conclusion] The experimental results show that MIP-PMAA/SiO2 have specific recognition selectivity,excellent binding affinity and elution property for ractopamine.关键词
表面分子印迹/莱克多巴胺/表征/吸附性能Key words
surface molecular imprinting/ ractopamine/ characterization/ adsorption characteristics引用本文复制引用
杨挺,吴银良,李存,赵健,陈国,吕燕..莱克多巴胺表面分子印迹材料的制备、表征及吸附性能[J].中国农业科学,2013,46(6):1256-1262,7.基金项目
国家自然科学基金项目(31072172)、宁波市农业与社会发展项目(2010C10008) （31072172）
