中国实用妇科与产科杂志2013，Vol.29Issue(6)：450-453,4.
5-氮杂胞苷诱导脐带间充质干细胞向成肌细胞分化研究
Umbilical cord mesenchymal stem cell was induced into myoblast with 5-azacytidine
摘要
Abstract
Objective Whether could human umbilical cord mesenchymal stem cell (HuMSC) differentiate into myoblast.Methods HuMSCs being isolated by adherent culture,checking surface markers by flow cytometry,observation of cells ultrastructural with transmission electron microscopy and atomic force electron microscopy,5-Aza inducting HuMSCs to myoblast differentiation,RT-PCR and Western Blot detecting myoblast specific genes and protein expression respectively.Results Stem cells could isolate from umbilical cord with adherent culture.HuMSCs don 1 express CD31,CD45,CD34,strongly express CD29,CD90,CD44,CD105,and extremely low express HLA-DR,CD40.Expression of myoblast special genes and proteins was significantly higher between experimental group and control group.Conclusion Adherent culture was a reliable and economic method.5-Aza could induce HuMSCs to myoblast differentiation.关键词
脐带间质干细胞/结蛋白/重链肌球蛋白/5-氮杂胞苷Key words
human umbilical cord mesenchymal stem cells /desmin /heavy chain myosin/5-azacytidine分类
医药卫生引用本文复制引用
蓝建发,罗新,宋泓,石海燕,郭红宇..5-氮杂胞苷诱导脐带间充质干细胞向成肌细胞分化研究[J].中国实用妇科与产科杂志,2013,29(6):450-453,4.基金项目
国家自然科学基金(81070459) （81070459）
