中国兽医科学2013，Vol.43Issue(6)：583-587,5.
猪圆环病毒2型ORF3基因的表达及其表达产物免疫小鼠后致细胞因子变化的研究
Expression of PCV2 ORF3 gene and study on cytokine profiles induced by the expressed ORF3 protein immunization in mice
王亚军 1杨顺利 2才学鹏3
作者信息
- 1. 甘肃农业大学动物医学院,甘肃兰州 730070
- 2. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室,甘肃兰州730046
- 3. 中国动物疫病预防控制中心,北京 100125
- 折叠
摘要
Abstract
To research the profiles changes of cytokine in the immunized mice with porcine circovirus type 2(PCV2) ORF3 protein,the specific primers were designed according to the published sequences of ORF3 gene of PCV2.The complete DNA fragment encoding ORF3 protein was obtained from viral DNA of PCV2 FJ948167 strain by PCR and the ORF3 gene was cloned into the prokaryotic expression vector pSMK-sumo.The resultant recombinant plasmid was named as ps-ORF3;and it was then transformed into BL21(DE3 RIL strain).SDS-PAGE and Western-blot analyses showed that the aim protein was expressed when it was induced by 0.5 mmol/L IPTG.The recombinant ORF3 protein was soluble and in molecular mass of 32 ku.Mice were vaccinated with the purified recombinant protein.The concentrations of IFN-y,IL-4,IL-10 and IL-12 in the peripheral blood were detected by a sandwich ELISA.In result,when compared with the control group,the content of IL-4 was increased significantly,but the content of IFN-γ,IL-10 and IL-12 has no significant changes.The result suggested that ORF3 protein plays a certain role in the humoral immunity in mice.关键词
猪圆环病毒2型/ORF3基因/γ-干扰素/白细胞介素4/白细胞介素10/白细胞介素12Key words
porcine circovirus type 2(PCV2)/ORF3 gene/IFN-γ/IL-4/IL-10/IL-12分类
农业科技引用本文复制引用
王亚军,杨顺利,才学鹏..猪圆环病毒2型ORF3基因的表达及其表达产物免疫小鼠后致细胞因子变化的研究[J].中国兽医科学,2013,43(6):583-587,5.
