中国兽医科学2013，Vol.43Issue(6)：599-604,6.
日本血吸虫dysferlin基因的克隆及重组蛋白免疫原性的分析
Cloning of dysferlin gene of Schistosoma japonicum and immunogenicity analysis of recombinant dysferlin protein
摘要
Abstract
To evaluate immunogenicity of recombinant Schistosoma japonicum dysferlin protein (rSjDF),SjDF gene was amplified by PCR and then subcloned into the plasmid pET-28a(+) to construct a recombinant expression plasmid pET-28a (+)-SjDF.The recombinant protein rSjDF was expressed in Escherichia coli.The purified recombinant protein was used to immunize BALB/c mice emulsified with ISA206 adjuvant three times to produce polyclonal antibodies.Indirect immunofluorescent analysis revealed that SjDF was mainly distributed on the tegument surface.Western-blot analysis showed that rSjDF was recognized by murine serum specific to this recombinant protein,indicating rSjDF had good immunogenicity.A high level of specific IgG,IgG1 and IgG2a antibodies as well as IL-2,IL-4,IL-10,IL-12p70 and IFN-g cytokines were detected after the vaccination of rSjDF.The results suggest that the recombinant protein can induce stronger immune responses in mice.The study provides the foundations for screening the vaccine candidate for schistosomiasis.关键词
日本血吸虫/dysferlin/表膜蛋白/基因克隆/免疫应答Key words
Schistosoma japonicum / dysferlin/ tegument surface proteins/ gene cloning/ immune response分类
农业科技引用本文复制引用
熊雅念,魏梅梅,洪炀,黄莉妮,孟培培,艾德宙,韩艳辉,傅志强,林矫矫..日本血吸虫dysferlin基因的克隆及重组蛋白免疫原性的分析[J].中国兽医科学,2013,43(6):599-604,6.基金项目
国家自然科学基金资助项目(31172315) （31172315）
中国博士后科学基金项目(2012M510630) （2012M510630）
公益性行业(农业)科研专项(200903036) （农业）
上海科技发展基金项目(12140902700) （12140902700）
中国农业科学院院长基金项目(2013JB18) （2013JB18）
