国际医药卫生导报2013，Vol.19Issue(16)：2458-2461,4.DOI:10.3760/cma.j.issn.1007-1245.2013.16.004
应用MLPA分析技术快速产前诊断21-三体综合征
Study on application use of multiplex ligation-dependent probe amplification in rapid prenatal diagnosis of trisomy 21
摘要
Abstract
Objective To evaluate the effectiveness of existing multiplex ligation-dependent probe amplification (MLPA) as a method of rapid prenatal diagnosis for trisomy 21.Methods 181 cases of amniotic fluid samples were detected for chromosome aneuploidies by MLPA.The experimental results were compared with those of G-banding karyotype analysis.Results 181 cases of samples were detected by MLPA,among which 177 cases of normal samples and 4 cases of chromosome aneuploidies were found,including 3 cases of trisomy 21,1 case of X-monomer.This result was consistent with the result of G-banding karyotype analysis.Detection results of MLPA achieved 99.4％ in 24 hours.Conclusion As a rapid prenatal diagnosis technology for trisomy 21,MLPA has good specificity and application value.关键词
多重连接依赖式扩增技术/快速产前诊断/21-三体综合征Key words
Multiplex ligation-dependent probe amplification (MLPA)/ Rapid prenatal diagnosis/Trisomy 21引用本文复制引用
滕奔琦,郝秀兰,章钧,林俊伟,侯红瑛..应用MLPA分析技术快速产前诊断21-三体综合征[J].国际医药卫生导报,2013,19(16):2458-2461,4.基金项目
广东省科技计划项目(2009B060700107) （2009B060700107）
