国际医药卫生导报2013，Vol.19Issue(16)：2462-2464,3.DOI:10.3760/cma.j.issn.1007-1245.2013.16.005
流式细胞术检测新生儿网织红细胞多参数参考范围的探讨
Study on the reference range of reticulocyte multi-parameter by flow cytometry in newborns
摘要
Abstract
Objective To establish the reference range of reticulocyte multi-parameter by using flow cytometry (FCM) in newborns.Methods A prospective study of neonatal infants admitted to neonatal general ward,ruled out infection,asphyxia and congenital malformation and admission time less than 12 hours of life from Dec.2012 to Jan.2013 was conducted.Blood reticulocyte percent (Ret％),reticulocyte maturity index (RMI) and reticulocyte mean fluorescence intensity on day 1,3,7 after birth were tested by using FCM.At the same time,Ret％ was tested by manual method and compared with the FCM.Results Of all 53 neonatal infants,36 were preterm infants with mean gestational age (35.08 ± 1.08) weeks,birth weight (2.35 ± 0.35) kg and 17 were term infants with mean gestational age (38.7 ± 0.89) weeks,birth weight (2.84 ± 0.64) kg.In preterm infants,Ret％ were (5.18 ± 1.34)％,(4.77 ± 1.45)％ and (1.84 ± 0.64)％respectively on day 1,3 and 7 after birth; RMI were (6.08 ± 3.14)％,(5.43 ± 3.08)％ and (22.54 ± 10.58)％ respectively; reticulocyte mean fluorescence intensity were (2626.06 ± 808.63),(2692.00 ± 865.87) and (4422.37 ± 1668.98) respectively.In term infants,Ret％ were (4.53 ± 0.98)％,(4.01 ± 1.62)％ and (1.55 ± 0.29)％ respectively on day 1,3 and 7 of life; RMI were (11.17 ± 6.31)％,(8.66 ± 4.49)％ and (24.09 ± 5.38)％; reticulocyte mean fluorescence intensity were (3522.50 ± 1398.45),(3888.00 ± 1814.06) and (4731.53 ± 1771.59).Compared with preterm infants,RMI and reticulocyte mean fluorescence intensity were significantly higher on day 1,3 of life in term infants (P＜0.05).However,there was no statistical significant difference in Ret％,RMI and mean fluorescence intensity on day 7 after birth in two groups.Conclusion FCM reticulocyte multi-parameter analysis in different gestational age and time of life can understand more information related to change rule of reticulocyte and bring us to the new research orientation and prospect in differential diagnosis,treatment and monitoring in neonatal anemia.关键词
流式细胞术/新生儿/网织红细胞Key words
Flow cytometry/ Neonate/ Reticulocyte引用本文复制引用
李月凤,陈睿,徐芬,朱传瑞,宫学雷,倪滨瑜,熊礼宽..流式细胞术检测新生儿网织红细胞多参数参考范围的探讨[J].国际医药卫生导报,2013,19(16):2462-2464,3.基金项目
广东省科技计划项目(2010152) （2010152）
