吉林中医药2013，Vol.33Issue(7)：726-728,3.
胡黄连总苷中胡黄连苷Ⅰ、Ⅱ质量分数测定
Content Determination of Picroside Ⅰ and Ⅱ in Total Iridoid Glucosides of Picrorhiza Scrophulariiflora
摘要
Abstract
Objective To establish the method for the content determination of picroside Ⅰ,Ⅱ and total glucosides of Picrorhiza.Methods HPLC was used to determine the content of picroside Ⅰ,Ⅱ and total glucosides of Picrorhiza.The liquid phase included acetonitrile and 0.5％ acetic acid (15.5:84.5).The flow rate was set to 1.2 mL/min with the column temperature at 35 ℃ and the detecting wavelength at 275 nm.Results the calibration curve was linear in the range of 0.128 0 ～ 1.280 0μg(r =0.999 6) of picroside Ⅰ and 0.250 6 ～ 2.506 0μg(r =0.999 6) of picroside Ⅱ,respectively.The average recovery was 98.59 ％ and 97.33 ％ with RSD at 1.57 ％ and 1.35 ％,respectively.Conclusion The method is sensitive,accurate,specific,and can be used for quality control of total glucosides of Picrorhiza.关键词
胡黄连总苷/胡黄连苷Ⅰ/胡黄连苷Ⅱ/胡黄连Key words
Picroside Ⅰ /Picroside Ⅱ /The total glucosides of Picrorhiza/content determination分类
医药卫生引用本文复制引用
侯文彬,周福军,单淇,刘昌孝..胡黄连总苷中胡黄连苷Ⅰ、Ⅱ质量分数测定[J].吉林中医药,2013,33(7):726-728,3.基金项目
国家科技支撑计划课题:中药天然药成药性关键技术及其应用(2007BAI41B06). （2007BAI41B06）
