南京医科大学学报（自然科学版）2013，Vol.33Issue(7)：873-876,4.DOI:10.7655/NYDXBNS20130703
顺铂诱导SPC-A1人肺腺癌细胞凋亡过程中miR-27a的表达及意义
Expression changes and significance of miR-27a in cisplatin-induced apoptosis of human lung adenocarcinoma cell line SPC-A1
摘要
Abstract
Objective:To investigate the expression changes of miR-27a in human lung adenocarcinoma cell line SPC-A1 after cisplatin chemotherapy in virto,and to discuss its relationship with cell apoptosis and clinical significance.Methods:SPC-A1 cells were cultured in vitro and treated with DDP at 2.5 μg/ml for 12,24,48 and 72 h.Cell morphology was observed by phase contrast microscope,as well as cell apoptosis was detected by flow cytometry; expression changes of miR-27a both in cultural supernatants and SPC-A1 cells were analyzed by real-time PCR.Results:SPC-A1 cells showed morphological changes of apoptosis in the DDP (2.5 μg/ml) group.The apoptotic rates of SPC-A1 cells at 48 and 72 h in the DDP (2.5 μg/ml) group[(59.3 ± 2.5)％ and (76.4 ± 3.1)％,respectively] were higher than that in the control group (P ＜ 0.05).PCR analysis demonstrated that the levels of miR-27a were significantly increased in the DDP (2.5 μg/ml) group both in cultural supernatants and SPC-A1 cells compared to the level of the control group (P ＜ 0.05).Conclusion:The level of miR-27a raised in human lung adenocarcinoma cell line SPC-A1 after cisplatin chemotherapy in virto.Quantitative analysis of miR-27a may have important value for chemotherapy monitoring of lung adenocarcinoma.关键词
顺铂/肺癌SPC-A1/细胞凋亡/miR-27aKey words
cisplatin/ SPC-A1 / cell apoptosis/ miR-27a分类
生物科学引用本文复制引用
朱善军,韩月,徐娟,谢而付,陈丹,张美娟,张燕,娄鉴芳,曹艳..顺铂诱导SPC-A1人肺腺癌细胞凋亡过程中miR-27a的表达及意义[J].南京医科大学学报（自然科学版）,2013,33(7):873-876,4.基金项目
国家自然科学基金资助(81272324,81000754,81101322,81201359) （81272324,81000754,81101322,81201359）
江苏省实验诊断学重点实验室基金(XK201114) （XK201114）
江苏高校优势学科建设工程资助 （）
