南京医科大学学报（自然科学版）2013，Vol.33Issue(7)：932-936,5.DOI:10.7655/NYDXBNS20130716
白细胞介素-10对冈田酸诱导的阿尔茨海默病模型大鼠的保护作用
Interleukin-10 prevents rats from okadaic acid-induced Alzheimer's disease
摘要
Abstract
Objective:To explore the protective effects of interleukin-10 (IL-10) pretreatment on the behavioral and molecular biochemical changes of Alzheimer'disease (AD) rat models and its possible mechanism.Methods:Okadaic acid (OA) was microinjected into the hippocampus of rats to establish the AD rat models.The escape latency of rats was tested by water maze.Western-blot method was used to detect the expression of protein phosphatase 2A (PP2A) and amyloid precursor protein (APP) in the hippocampus.Simultaneously,the ratio of CD4+ cells to the CD8+ cells in the mesenteric lymph node cells was examined by flowcytometry.Results:The latent period in water maze of rats that were injected with OA in the hippocampus was significantly increased,and the latent period of the rats with high concentration of OA injection was longer than that of the rats with low concentration of OA injection.The PP2A protein expression in hippocampus after the OA injection was down-regulated,and the inhibitory effect of OA on PP2A protein expression was enhanced as the OA concentrations increased.Conversely,APP protein expression in hippocampus was increased with the elevated concentrations of OA injected in the hippocampus.After IL-10 and OA were injected into the rat hippocampus respectively,the latent period of water maze was significantly lower than that of the AD rats only with the OA injection.IL-10 reversed the inhibitory effect of OA on PP2A expression and alleviated the augument of APP;furthermore,IL-10 reversed the increasing of CD4+/CD8+ induced by OA and caused the CD4+/CD8+ return to the control level.Conclusion:AD-like changes can be induced by the microinjection of OA into the hippocampus.IL-10 can reverse the AD-like changes induced by OA,and the effects of IL-10 are dose-dependent and also closely related to its inhibition of inflammatory reaction.关键词
阿尔茨海默病/冈田酸/蛋白磷酸酯酶2A/淀粉样蛋白前体/白细胞介素-10/CD4+/CD8+Key words
Alzheimer's disease/ okadaic acid /protein phosphatase 2A/amyloid precursor protein/interleukin-10/ CD4+/CD8+分类
医药卫生引用本文复制引用
李冰,毛丰,方小霞,邱一华..白细胞介素-10对冈田酸诱导的阿尔茨海默病模型大鼠的保护作用[J].南京医科大学学报（自然科学版）,2013,33(7):932-936,5.基金项目
江苏省自然科学基金项目(BK2011386) （BK2011386）
南通大学自然科学基金(11Z003,12Z003) （11Z003,12Z003）
