南京医科大学学报（自然科学版）2013，Vol.33Issue(7)：1019-1023,5.DOI:10.7655/NYDXBNS20130735
流感疫苗脂质体干粉制备方法对其免疫原性影响研究
A study on immunogenicity of influenza vaccine lyophilized liposome prepared by different methods
摘要
Abstract
Objective:To evaluate the immunity effect of H1N1 influenza vaccine prepared by film-dispersion and freeze-thawing method,and to provide expermental foundation for attenuated live vaccine liposome preparation.Methods:Experimental mice were divided into the influenza vaccine non-liposome group,the film-dispersion prepared H1N1 influenza vaccine liposome group,the freeze-thawing lyophilized prepared H1N1 influenza vaccine liposome group,the positive control group and the negative control group (n=5).4 μg and 6 μg hemagglutinin of H1N1 subtype per mouse were tracheally delivered to the mice for the non-liposome group and the lyophilized liposome groups,with the same dose intraperitoneally delivered groups as the positive control,and the PBS intraperotoneal injection group as the negative control.After 7,14 and 28 d of immunization,serum levels of HA antibodies were measured by the hemagglutination-inhibition method,and serum cytokine levels were measured by the ELISA method.Results:By pulmonary injection of the two liposome groups,the antibody titers of 6 μg dose groups were higher than those of 4 μg dose groups (P ＜ 0.05).HA antibody titers of the two pulmonarily delivered liposome groups were higher than those of the pulmonarily (P ＜0.05) and intraperitoneally (P ＜ 0.01) injected non-liposome group.Antibody titers of the film-dispersion liposome group for both doses were higher than those of the freeze-thawing lyophilized liposome group.IL-2 and IFN-γproduced by the pulmonary injected liposome groups were higher than those of the intraperitoneally delivered non-liposome group.Conclusion:The humeral and cellular immunities could be effectively induced by pulmonarily delivered vaccine liposomes; The film-dispersion method group is better than the freeze-thawing lyophilized group in terms of immunity strength.For the freeze-thawing lyophilized prepared H1N1 influenza vaccine liposome group,the immune response occurs at 14 d after vaccination,white the titer ratio is only 1.8 for normal vaccinated flu vaccine.The freeze-thawing lyophilized liposome vaccine also stimulates higher levels of cytokines.关键词
流感疫苗/冻干脂质体/肺部给药/血凝抑制试验/细胞因子Key words
influenza vaccine/ lyophilized liposome/ pulmonary delivery/ hemagglutination-inhibition test/ cytokines分类
医药卫生引用本文复制引用
陈建雯,马波,林华,乔建斌,王礼燕,鲁卫东..流感疫苗脂质体干粉制备方法对其免疫原性影响研究[J].南京医科大学学报（自然科学版）,2013,33(7):1019-1023,5.基金项目
云南省科技厅应用基础研究面上项目(2008ZC113M) （2008ZC113M）
