畜牧与兽医2013，Vol.45Issue(1)：6-9,4.
连续液相萃取法提取豆类丝核菌培养液中苦马豆素
Extraction of swainsonine from Rhizoctonia lguminicola culture by the continuous liquid/liquid procedure
摘要
Abstract
This experiment is conducted to find an effective method for the extraction of swainsonine from Rhizoctonia leguminicola culture.R.leguminicola were cultured on Czapek's medium,and the culture supernathant was collected after 14d.A kind of straw yellow crudes was obtained using the ultrasonic-water extraction,chloroform extraction and continuous liquid/liquid extraction procedure by dichloromethane for 60-70h.Then 5mg of white crystals was further obtained by decompression at 90℃.The existence of swainsonine was demonstrated by thin layer chromatography (TLC) and gas chromatography (GC).It suggests that swainsonine can be extracted from R.leguminicola culture by the continuous liquid/liquid extraction procedure.关键词
超声波-水提法/连续液相萃取法/豆类丝核菌/苦马豆素Key words
ultrasonic-water extraction/ continuous liquid/liquid extraction/ Rhizoctonia leguminicola/ swainsonine分类
生物科学引用本文复制引用
罗颉,杨鸣琦,郭良,刘宸铄..连续液相萃取法提取豆类丝核菌培养液中苦马豆素[J].畜牧与兽医,2013,45(1):6-9,4.基金项目
国家自然科学基金(30270979) （30270979）
陕西省自然科学基金(2003C119). （2003C119）
