畜牧与兽医2013，Vol.45Issue(1)：25-28,4.
牛源犬新孢子虫NcSRS2-NcGRA7融合基因的克隆与生物信息学分析
Cloning and bioinformatics analysis of NcSRS2-NcGRA7 fusion gene of bovine Neospora caninum
摘要
Abstract
In order to understand the biological characteristic of NcSRS2-NcGRA7 fusion gene of bovine Neospora Caninum,genomic DNA of N.caninum was extracted.The surface protein gene NcSRS2 and granule protein gene NcGRA7 were amplified by PCR,respectively.Nc-SRS2-NcGRA7 fusion gene was synthesized by SOE-PCR.The NcSRS2-NcGRA7 cloning vector was constructed and identified by PCR amplification,double digestion and bioinformatic analysis.The results showed that the sizes of NcSRS2,NcGRA7 and NcSRS2-NcGRA7 were 1061 bp,364 bp and 1482 bp,respectively.The cloning vector was demonstrated to be correct by PCR and double digestion.Sequencing analysis showed that the nucleotide sequences of NcSRS2 and NcGRA7 shared 99％ homology with those published in GenBank (AF061249,AF176649).Prediction analysis by DNAman software indicated that the antigen index of NcSRS2-NcGRA7 was high.The main secondary structure of the fusion protein was mainly composed of α-helix and β-sheet.The tertiary structure analysis showed that the protein could fold into two independent regions connected with a linker.It indicated that the functions of NcSRS2 and NcGRA7 were not affected by each other.This study applies a foundation for the immunological functions of NcSRS2-NcGRA7 fusion protein of bovine N.caninum.关键词
牛/犬新孢子虫/NcSRS2-NcGRA7/克隆/生物信息学Key words
bovine/ Neospora caninum/ NcSRS2-NcGRA7/ cloning/ bioinformatics analysis分类
农业科技引用本文复制引用
贾立军,张守发,谷振甲..牛源犬新孢子虫NcSRS2-NcGRA7融合基因的克隆与生物信息学分析[J].畜牧与兽医,2013,45(1):25-28,4.基金项目
国家自然科学基金(31160501) （31160501）
吉林省青年科研基金(201201076) （201201076）
延边大学基金(延大科合字2011第37号). （延大科合字2011第37号）
