昆虫学报2013，Vol.56Issue(6)：605-611,7.
麦红吸浆虫蜕皮激素受体(EcR)基因的克隆与表达分析
Molecular cloning and expression analysis of an ecdysone receptor (EcR) gene in the wheat midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae)
摘要
Abstract
In order to study the function of ecdysone receptor in the diapause of Sitodiplosis mosellana,the full-length cDNA sequence of an ecdysone receptor gene was amplified by using reverse transcriptionpolymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE),and the expression of the EcR gene was analyzed using real-time quantitative PCR (qPCR).The full-length cDNA sequence was named as SmEcR (GenBank accession number:KC491135).Its open reading frame (ORF) is 1 386 bp in length,encoding a 461-amino-acid protein,with a calculated molecular weight of 52.90 kD and the theoretical isoelectric point of 6.24.Multiple sequence alignment revealed that the deduced amino acid sequence of SmEcR has high identity with EcRs from other insect species,especially with that of Bradysia coprophila (92％).Real-time quantitative PCR showed that SmEcR transcripts were detected in all diapause and developmental stages.The expression of SmEcR was significantly different among different diapause stages,reaching the highest level in November and the lowest level in December.The larvae collected from wheat heads had a lower expression level of SmEcR while the adults had the highest SmEcR transcripts.This study lays the foundation for the further functional study of SmEcR in diapause regulation in S.mosellana.关键词
麦红吸浆虫/蜕皮激素受体/基因克隆/滞育/表达分析Key words
Sitodiplosis mosellana/ecdysone receptor (EcR)/gene cloning/diapause/expression analysis分类
生物科学引用本文复制引用
马康生,李伯辽,陈浩,仵均祥..麦红吸浆虫蜕皮激素受体(EcR)基因的克隆与表达分析[J].昆虫学报,2013,56(6):605-611,7.基金项目
高等学校博士学科点专项科研基金(20100204110006) （20100204110006）
国家公益性行业(农业)科研专项(200903004) （农业）
