临床肝胆病杂志2013，Vol.29Issue(7)：525-528,4.DOI:10.3969/j.issn.1001-5256.2013.07.012
肝癌组织中DNA甲基转移酶基因表达的分析
DNMT expression in hepatocellular cancer
摘要
Abstract
Objective To investigate the differential expression of DNA methyltransferase (DNMT) in hepatocellular carcinoma (HCC).Methods DNMT mRNA expression was measured by fluorescence quantitative-PCR in specimens of cancerous tissues (n =44) and case -matched cirrhotic (n =35) and non-cancerous,non-cirrhotic (normal; n =44) liver tissues from 44 HCC patients with different tumor stages.Results The mean mRNA levels of the four DNMT types tested (1,2,3A,3B) were higher in cancerous and cirrhotic tissues than in adjacent normal tissues.The DNMT1 elevated expression reached statistical significance (vs.adjacent normal tissues,P ＜0.01 for cancerous tissue and P =0.02 for cirrhotic tissue),unlike the DNMT2 elevated expression which did not reach statistical significance (vs.adjacent normal tissues,P =0.12 for cancerous and P =0.35 for cirrhotic tissues).Both DNMT3 isoforms,A and B,were significantly higher than the levels detected in the adjacent healthy tissues (DNMT3A:P ＜ 0.01 for cancerous and P =0.01 for cirrhotic tissue; DNMT3B:P =0.03 for cancerous and P ＜ 0.05 for cirrhotic tissue).Stratification analysis of the four DNMTs mRNA expression levels according to patient sex,tumor staging,and tumor differentiation revealed no correlations (DNMT1:P =0.23; DNMT2:P =0.45; DNMT3A:P =0.32; DNMT3B:P =0.36).However,trends were found for increased DNMTs mRNA expression levels and male sex,poor tumor differentiation,and high tumor stage.Conclusion The mRNA expressions of DNMT1,DNMT3A,and DNMT3B are higher in cancerous and cirrhotic tissues from HCC patients than in their adjacent non-cancerous,non-cirrhotic liver tissues.Expression of DNMTs may be associated with pathogenesis of HCC or HCC associated with cirrhosis.关键词
DNA修饰甲基酶类/肝肿瘤/肝硬化Key words
DNA modification methylases/ liver neoplasms/ liver cirrhosis分类
医药卫生引用本文复制引用
李海平,余宗涛,范金波,高波,张吉才..肝癌组织中DNA甲基转移酶基因表达的分析[J].临床肝胆病杂志,2013,29(7):525-528,4.基金项目
湖北省教育厅重大项目资助(Z20082401) （Z20082401）
