林业科技开发2013，Vol.27Issue(4)：80-84,5.DOI:10.3969/j.issn.1000-8101.2013.04.021
蝴蝶兰丛生芽快繁体系的建立
Mass propagation of clustered shoots of Phalaenopsis
摘要
Abstract
With an axillary bud of flower stalks as explants,sterilization methods,medium types,hormone concentration and browning control were studied.The results showed that the 2-3 cm cutting segments with an axillary bud of flower stalks were soaked into 0.1％ HgC12 for 15 min after series of pre-treatment.The aseptic rate could reach 90％.The suitable medium for primary culture was MS or 1/2MS +6-BA 3.0 mg/L.The suitable medium for subculture was 1/2MS +6-BA 7.5 mg/L + NAA 1.0 mg/L.The suitable medium for rooting was 1/2MS + NAA 1.0 mg/L + AC 1.0 g/L + banana pulp 60 mg/L.A method for mass propagation was found through inducing clustered shoots.关键词
蝴蝶兰/花梗腋芽/激素浓度/丛生芽Key words
Phalaenopsis/ axillary bud/ hormone concentration/ clustered shoots引用本文复制引用
谭鹏鹏,彭方仁,江荣翠,尚杨娟..蝴蝶兰丛生芽快繁体系的建立[J].林业科技开发,2013,27(4):80-84,5.基金项目
国家"十二五"科技支撑计划课题子项目(编号:2012BAD21B00). （编号:2012BAD21B00）
