南京医科大学学报（自然科学版）2013，Vol.33Issue(8)：1066-1069,4.DOI:10.7655/NYDXBNS20130808
胃旁路术对非肥胖2型糖尿病大鼠肝脏葡萄糖激酶表达的影响
Effect of gastric bypass on glucokinase expression in non-obese type 2 diabetid rat liver
摘要
Abstract
Objective:To investigate the effect of gastric bypass on the expression of liver glucokinase (GCK) in non-obese GotoKakizaki (GK) type 2 diabetic rats.Methods:Twenty male GK rats and twenty male Wistar rats were randomly divided into four groups:GK operation group (GO group),GK sham operation group (GS group),Wistar operation group (WO group),Wistar sham operation group (WS group).There were 10 rats in each group.The fasting blood glucose and serum insulin levels were measured before operation and on the 1st,4th and 8th week after the operation.Liver tissues were harvested 8 weeks postoperatively and reverse transcriptase polymerase chain reaction was used to detect liver GCK mRNA expression after the operation.Results:The fasting blood glucose of GO group decreased as early as the 1st week after surgery,which decreased from preoperative (8.73 ± 1.30) mmol/L to (6.84 ± 0.89),(6.27 ± 0.93),(5.86 ± 0.57) mmol/L on the 1st,4th and 8th week postoperation (P ＜ 0.05).The insulin level remained consistently higher in GO group with respect to GS group,but there was no significant change during the follow-up period.On the 8th week after surgery,the liver GCK mRNA level in GO group were significantly higher than GS group (P ＜ 0.05).Conclusion:Gastric bypass can directly improve glucose metabolism in non-obese type 2 diabetic rats,the increased expression of liver tissue GCK mRNA might contribute to the hypoglycemic effect of the surgery.关键词
胃旁路术/2型糖尿病/GK大鼠/胰岛素/葡萄糖激酶Key words
gastric bypass/ type 2 diabetes mellitus/ Goto-Kakizaki rats/ insulin/ glucokinase分类
医药卫生引用本文复制引用
焦雪花,马向华,卢姗,田婷,张媛媛..胃旁路术对非肥胖2型糖尿病大鼠肝脏葡萄糖激酶表达的影响[J].南京医科大学学报（自然科学版）,2013,33(8):1066-1069,4.基金项目
江苏省卫生厅医学科技发展基金(H201202) （H201202）
