山东医药2013，Vol.53Issue(23)：27-29,3.DOI:10.3969/j.issn.1002-266X.2013.23.008
噬菌体展示肽库在乳腺癌血清肿瘤标志物筛选中的应用
Application of phage display peptide library in screening of the tumor markers in the serum of breast caner
摘要
Abstract
Objective To find the new specific tumor marker for breast cancer through screening the phage display peptide library in the serum of patients with breast cancer.Methods Three rounds of subtract biopanning were carried out.ELISA was used to confirm the specificity ofphages clones.Results After three rounds of screening,the enrichment ratio was increased.A phage with high specificity was found and confirmed in the serum of 47 cases of breast cancer patients and normal people by ELISA.The two phage clone contained the same sequence after DNA sequencing,which was QVSAEHKVQGFW.Conclusions A high affinity dodecapeptide specific for the serum of breast cancer was obtained through phage display peptide library.It can be used for the next step in tumor marker of breast cancer.关键词
噬菌体肽库/噬菌体展示技术/多肽/肿瘤标志物/减性筛选Key words
phage peptide library/ phage display technology/ polypeptide/ tumor marker/ subtract biopanning分类
医药卫生引用本文复制引用
贺文,曾健,贺海平,徐佳,李富..噬菌体展示肽库在乳腺癌血清肿瘤标志物筛选中的应用[J].山东医药,2013,53(23):27-29,3.基金项目
广西壮族自治区科学技术厅自然科学基金资助项目(0991232). （0991232）
