骨碎补提取物对骨髓间充质干细胞向成骨细胞分化及Cbfα1表达的影响OACSTPCD
Effect of drynaria extract on the osteogenic differentiation of BMSCs and expression of Cbfα1
目的 探讨中药骨碎补对大鼠骨髓间充质干细胞增殖分化及核心结合因子α1(Cbfα1)表达的影响.方法 采用中药干预培养细胞的方法,利用骨碎补单味药物与培养基联合培养大鼠骨髓间充质干细胞(BMSCs),分为标准培养基组(无血清培养基)、条件培养基组(地塞米松1×10-8 mol/L、β-甘油磷酸钠10 mol/L、VitC 50 μg/mL)、中药1组(骨碎补提取液20 mg/mL)、中药2组(骨碎补提取液2 mg/mL)、中药3组(骨碎补提取液0.…查看全部>>
Objective To investigate the effect of drynaria extract on the proliferation and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) and expression of Cbfα1.Methods With the method of interference of Chinese medicine cell culture,single drynaria combined with medium was used to coculture the rat BMSCs.The rat BMSCs were divided into 5 groups:the standard medium group (serum-free culture medium),conditioned medium group(dexamethasone 1 × 10-8 mo…查看全部>>
刘国岩;徐展望;徐琬梨
山东中医药大学附属医院,济南250014山东中医药大学附属医院,济南250014山东中医药大学
医药卫生
骨碎补骨髓间充质干细胞Cbfα1成骨能力大鼠
drynaria fortunei bone marrow mesenchymal stem cells core binding factor A1 osteogenic potential rats
《山东医药》 2013 (25)
10-12,3
山东省中医药科技发展计划项目(2005-172).
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