山东医药2013，Vol.53Issue(27)：1-3,3.DOI:10.3969/j.issn.1002-266X.2013.27.001
枯草芽孢杆菌芽孢佐剂免疫途径及效果的动物实验观察
Experimental study on immunization route and effect of bacillus subtilis spores as mucosal adjuvant
摘要
Abstract
Objective To study the application effect of bacillus subtilis spores which displayed functional domains of immunoglobulin binding protein(FDIBP) on surface as mucosal adjuvant in different immunization routes.Methods Bacillus subtilis spores displaying FDIBP,Escherichia coli heat-labile enterotoxin (LT) and hIgG as adjuvants were incubated for preparing the vaccines.Then the BALB/c mice were separately conducted by intragastric administration and intranasal immunization.5 weeks later,the eyeballs were picked out to get blood.The mice were killed by cervical dislocation to collect flushing fluids of pulmonary alveolar,small intestine and vagina.Then the level of hIgG-specific antibody IgA in serum,lung,intestine and vagina flushing fluids and the levels of hIgG-specific antibody IgG,IL-2,IL-4,IL-5 and IFN-γ were detected by ELISA.Results Compared with those without adjuvants,bacillus subtilis spores adjuvants through intranasal immunization effectively enhanced the immunity of mice to produce antigen-specific antibodies IgA and IgG (P ＜0.01),but failed to effectively up-regulate serum levels of IL-2,IL-4,IL-5 and IFN-γ; there were no significant changes of the specific immunity antibody and cytokines in intragastric administration (all P ＞ 0.05).Conclusion The spores displaying FDIBP can be used as adjuvant in intranasal immunization and effectively stimulate the body to produce special mucosal immune response.关键词
黏膜佐剂/免疫球蛋白结合蛋白/枯草芽孢杆菌芽孢Key words
mucosal adjuvant/immunoglobulin binding protein/bacillus subtilis spores分类
医药卫生引用本文复制引用
李志会,李鹏,岳盈盈,宋楠楠,纪璇,曹银光,孟红..枯草芽孢杆菌芽孢佐剂免疫途径及效果的动物实验观察[J].山东医药,2013,53(27):1-3,3.基金项目
国家自然科学基金资助项目(81000720) （81000720）
山东省自然科学基金资助项目(2009ZRC03096). （2009ZRC03096）
