山东医药2013，Vol.53Issue(27)：23-25,3.DOI:10.3969/j.issn.1002-266X.2013.27.009
乌梅丸对TNBS诱导的溃疡性结肠炎大鼠脾脏组织β-arrestin1表达的影响
Effect of Wumeiwan on the expression of β-arrestin1 in the spleen tissue of rats with TNBS-induced ulcerative colitis
摘要
Abstract
Objective To observe the effect of Wumeiwan on the expression of β-arrestin1 in the spleen tissue of rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced ulcerative colitis.Methods Fifty-six SD rats were randomly divided into the control group,colitis model group,mesalazine group and Wumeiwan group (14 rats in each group).Apart from the control group,rats in the other three groups were induced to experimental colitis by 2,4,6-trinitrobenzenesulfonic acid.And 2 days later,rats in the control group and colitis model group were administered intragastrically with normal saline at a dose of 3 mL,while rats in the mesalazine group and Wumeiwan group were intragastrically given mesalazine (50 g/L) and Wumeiwan (0.51 g/L) at a dose of 3 mL,respectively.All rats were treated for 15 d.Spleen tissue samples were taken to detect the expression of mRNA of β-arrestin1 by RT-PCR and the expression of β-arrestin1 protein by Western blot.Results Compared with the control group,the expression of β-arrestin1 mRNA and β-arrestin1 protein was significantly increased in the colitis model group (all P ＜ 0.05) ; compared with the colitis model group,the expression of the mRNA and protein of β-arrestin1 in the Wumeiwan and mesalazine groups was significantly decreased (all P ＜ 0.05).Conclusion Wumeiwan can down-regulate the expression of the mRNA and protein of β-arrestin1 in the spleen tissue of rats with TNBS-induced ulcerative colitis.关键词
乌梅丸/溃疡性结肠炎/β-arrestin1Key words
Wumeiwan/ulcerative colitis/β-arrestin1分类
医药卫生引用本文复制引用
柯琴梅,吴霁,范恒..乌梅丸对TNBS诱导的溃疡性结肠炎大鼠脾脏组织β-arrestin1表达的影响[J].山东医药,2013,53(27):23-25,3.基金项目
国家自然科学基金资助项目(81072944) （81072944）
湖北省自然科学基金资助项目(2011CDB386). （2011CDB386）
