上海海洋大学学报2013，Vol.22Issue(4)：481-487,7.
缢蛏丝氨酸蛋白酶基因的序列特征及其表达分析
Molecular characteristics and expression analysis of serine protease from Sinonovacula constricta
摘要
Abstract
The serine protease with clip domain is a new serine protease family and plays an important role in innate immunity.One EST sequence with high homology with serine protease gene of other species was found from the cDNA library of Sinonovacula constricta and then the complete ORF and 3'UTR sequence were obtained by PCR.The 5' UTR sequence was got by 5' RACE.The cDNA of this gene was 1 228 bp,which consists of a 66 bp 5' untranslated region (UTR),a 1 002 bp open reading frame (ORF) and a 160 bp 3' UTR.The translated protein is composed of 333 amino acids containing a signal peptide.Sequence analysis of the protein revealed that the protein contained a clip domain and a Tryp_SPc domain.The three disulfide bonds were formed by six conserved cysteines in the clip domain and the catalytic triad (HDS) was contained in the Tyrp_SPc domain.This gene was designated as ScSP.The quantitative reverse transcriptase (qRTPCR) analyses showed that the ScSP can be expressed in six tissues.The expression level of ScSP gene was highest in liver,then in gonad,but lowest in water pipe,mantle and gill.The expression of ScSP gene in liver tissue was up-regulated at 4h and 8h following the challenge with Vibrio anguillarum.These results indicated that ScSP is a new serine protease with clip domain and might be involved in innate immunity,which contributes to understanding the structure and function of serine protease in the future.关键词
缢蛏/丝氨酸蛋白酶/序列分析/基因表达Key words
Sinonovacula constricta/ serine protease/ sequence analysis/ gene expression分类
农业科技引用本文复制引用
金凯,牛东红,王劦,李家乐..缢蛏丝氨酸蛋白酶基因的序列特征及其表达分析[J].上海海洋大学学报,2013,22(4):481-487,7.基金项目
国家高技术研究发展计划项目(2012AA10A400-3) （2012AA10A400-3）
国家自然科学基金(31101897) （31101897）
上海知识服务平台项目(ZF1206) （ZF1206）
