上海海洋大学学报2013，Vol.22Issue(4)：488-495,8.
牙鲆纤维蛋白原相关蛋白(FREP1)基因的克隆和表达分析
Cloning and expression analysis of a fibrinogen-related protein (FREP1) gene from Japanese flounder (Paralichthys olivaceus)
摘要
Abstract
Full-length cDNA FREP1 (fibrinogen-related protein) was cloned from larval Paralichthys olivaceus by the way of RACE-PCR,and 1 131 bp-length FREP1 cDNA with a 786 bp open reading frame encoded a putative protein containing a fibrinogen C-terminal domain signature (WWYSRCGSAGLNG).The whole amount in situ hybridization showed that FREP1 was only expressed in intestine of 2 dph and 5 dph (days post-hatching) larvae,but expressed in fin,skin,gill and intestine of 9 dph and 13 dph larvae.qRTPCR reveals that FREP1 was expressed mainly in fin,skin,gill and intestine of adult fish,but was expressed very lowly in liver,heart,kidney,spleen and muscle.If muscle expression was a control sample,compared with the other tissues the expression of FREP1 in fin and skin were highest (P ＜ 0.01).But compared with gill and intestine,the expression of FREP1 in heart,kidney,spleen,liver and muscle is much lower(P ＜0.05).Since it was mainly expressed in skin,gill and intestine,which was associated with innate immunity system,FREP1 may be an important member of innate immune system of P.olivaceus.关键词
牙鲆/纤维蛋白原相关蛋白基因/RACE-PCR/荧光定量Key words
Paralichthys olivaceus / FREP / RACE-PCR / qRT-PCR分类
农业科技引用本文复制引用
陈文君,鲍宝龙..牙鲆纤维蛋白原相关蛋白(FREP1)基因的克隆和表达分析[J].上海海洋大学学报,2013,22(4):488-495,8.基金项目
国家自然科学基金(30771668) （30771668）
