上海海洋大学学报2013，Vol.22Issue(4)：502-509,8.
日本文昌鱼ZP蛋白氨基酸序列多样性的初步研究
The preliminary study on the amino acid sequence diversity of ZP proteins from Branchiostoma japonicum
摘要
Abstract
ZP protein is a family of proteins which possess a conserved ZP domain and generally play an important role in the fertilization process.In this study,a new ZP cDNA from Branchiostoma japonicum,which codes 927 amino acid residues,was found.By using SMART software,it was confirmed that the ZP protein contains three domains (ZP Domain,Transmembrane Domain and Low Complexity Region).Furthermore,the sequence diversity of this ZP gene in B.japonicum population was analyzed.As a result,the ZP sequences amplified from 9 B.japonicum individuals are 2 481-2 784 bp,which code 826-927 amino acids.There are 55 variable cites (5.93％,55/927) identified by amino acid sequence alignment.9 and 101 amino acids fragment deletion in two individuals may be due to the diverse alternative splicing of ZP gene.In conclusion,the sequence diversity of ZP proteins may be correlated with its structure and function diversity.关键词
日本文昌鱼/ZP蛋白/ZP结构域/序列多样性Key words
Branchiostoma japonicum / ZP protein / ZP domain / sequence diversity分类
农业科技引用本文复制引用
王忠军,许强华..日本文昌鱼ZP蛋白氨基酸序列多样性的初步研究[J].上海海洋大学学报,2013,22(4):502-509,8.基金项目
国家海洋863计划项目(2008AA092602) （2008AA092602）
