色谱2013，Vol.31Issue(7)：691-696,6.DOI:10.3724/SP.J.1123.2012.12039
基于液相色谱-质谱联用技术的肺癌细胞代谢组学分析
Metabonomics study of lung cancer cells based on liquid chromatography-mass spectrometry
摘要
Abstract
The metabolic profiles of the polar metabolites and the non-polar metabolites in lung tumor cell lines H358,A549,HCC827,H1299,Calu-3,Calu-1,PC-9 and normal cell line MRC-5 were analyzed separately using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS).Partial least square discriminant analysis (PLS-DA) was used to process the metabolic data.The results showed that the metabolites of the lung cancer cell lines and normal cell line have significant differences.Further,10 polar metab-olites and 21 non-polar metabolites which had a significant contribution to classification were selected and preliminarily identified due to the accurate mass.Comparing with the normal cell line,the lung tumor cell lines present an abnormal metabolism in protein,fatty acid,and phospholipids.These results may provide important information for the early diagnosis of lung cancer.关键词
高效液相色谱-四极杆飞行时间质谱/偏最小二乘判别分析/代谢组学/肺癌Key words
high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS)/partial least squares discriminant analysis (PLS-DA)/metabonomics/lung cancer分类
化学化工引用本文复制引用
余欣尉,吴谦,吕望,王彦,马小琼,陈喆,阎超..基于液相色谱-质谱联用技术的肺癌细胞代谢组学分析[J].色谱,2013,31(7):691-696,6.基金项目
国家自然科学基金项目(21175092,21105064) （21175092,21105064）
国家重大科学仪器设备开发专项(2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210) （2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210）
浙江省科技厅公益性技术应用研究计划(2011C37014) （2011C37014）
浙江省卫生高层次创新人才培养工程专项经费. （）
