现代妇产科进展2013，Vol.22Issue(6)：437-440,4.
雌激素跨膜受体GPR30介导子宫内膜癌中IL-6/STAT3炎症信号通路的激活
Study on the expression of IL-6/STAT3 in endometrial cancer cells mediated by GPR30
摘要
Abstract
Objective:To study the expression of IL-6/STAT3 in endometrial carcinoma cell lines mediated by G-protein coupled receptor 30 (GPR30).Methods:The endometrial cancer cell lines Ishikawa and KLE were divided into 5 groups:Con,E2,G1,E2+G15 (GPR30 specific antagonist),G1 + G15.The expression of GPR30 was downregulated in endometrial cancer cells by transfection with shGPR30-pGFP-V-RS,a GPR30 antisense expression vector.The protein expression level of STAT3 in each group was determined by Western blot.The expression level of IL-6 was detected by ELISA.Result:The expression of STAT3 and the secretion of IL-6 in E2 and G1 group were significantly higher than those in the control group,E2 +G15 and G1 +G15 group(P＜0.05).Inhibition of GPR30 by RNA interference could reduce the expression of STAT3 and the secretion of IL-6 in E2 and G1 group.Conclusions:The E2 and G1 can promote the expression of IL-6/STAT3 in endometrial cancer cells.Downregulation of GPR30 can inhibit the expression of IL-6/STAT3 induced by E2 and G1 in endometrial cancer cells.Estrogen transembrane receptor GPR30 plays an important role in endometrial carcinoma by regulating the expression of IL-6/STAT3.关键词
子宫内膜癌/白介素6/GPR30/STAT3Key words
Endometrial carcinoma/ IL-6 / GPR30 / STAT3分类
医药卫生引用本文复制引用
蔡玲玉,贺银燕,席晓薇,张箴波,万小平..雌激素跨膜受体GPR30介导子宫内膜癌中IL-6/STAT3炎症信号通路的激活[J].现代妇产科进展,2013,22(6):437-440,4.基金项目
国家自然科学基金项目(No:81001154/H1621) （No:81001154/H1621）
