畜牧与兽医2013，Vol.45Issue(2)：7-12,6.
猪链球菌2型HA0609的分离及致病性研究
Isolation and pathogenicity of Streptococcus suis type 2 strain HA0609
摘要
Abstract
One bacterial strain was isolated from tonsillar specimen of a slaughtered healthy pig in Jiangsu province,and identified as Streptococcus suis type 2 based on morphological,cultural and biochemistry characteristics,and PCR detection of capsular polysaccharide (cps).The isolate was named as HA0609.The genes of seven virulence factors,including glutamate dehydrogenase (gdh),extracellular factor (epf),muramidase-released protein (mrp),suilysin (sly),fibronectin-binding protein (fpbs),inosine 5-monophosphate dehydrogenase gene (impdh) and virulence-associated sequence orf2,were detected by PCR amplification.Animal pathogenicity of HA0609 was compared with two virulent strains of S.suis type 2.The results showed that two mainly virulent factor genes sly and epf could not be detected in HA0609.And animal pathogenicity test showed that HA0609 could not cause piglets,rabbits and Balb/c mice to death.关键词
猪链球菌2型/扁桃体/分离/致病性Key words
Streptococcus suis type 2 / tonsillar specimen / isolation / pathogenicity分类
农业科技引用本文复制引用
吕立新,何孔旺,倪艳秀,俞正玉,茅爱华..猪链球菌2型HA0609的分离及致病性研究[J].畜牧与兽医,2013,45(2):7-12,6.基金项目
江苏省自然科学基金面上项目(BK2009327) （BK2009327）
江苏省自然科学基金重点项目(BK2010068) （BK2010068）
公益性行业(农业)科研专项经费(20080316-01) （农业）
江苏省农业科技自主创新探索性研究项目(CX(11)4040). （CX(11）
