产气荚膜梭菌多重PCR定型方法的建立及其应用OA北大核心CSTPCD
Establishment of a multiplex PCR method for Clostridium perfringens typing and its application
建立了多重PCR检测产气荚膜梭菌α、β、ε和ι毒素基因的方法.该方法具有良好的特异性和敏感性,只有产气荚膜梭菌呈现阳性,被检验的其他梭菌以及大肠杆菌、葡萄球菌均为阴性；将肉汤菌液样品10倍系列稀释后进行检测,检测灵敏度达到1.2× 104 CFU/mL.收集40份牛粪便样品,进行PCR检测,32份样品中成功扩增出589 bp的α毒素基因片段,阳性率为80％.结果显示,建立的多重PCR检测方法可取代血清中和试验,用于产气荚膜梭菌分型,同时表明A型产…查看全部>>
A multiplex PCR assay was developed to detect the alpha,beta,epsilon,and iota toxin genes of Clostridium perfringens.The specificity and sensitivity of the PCR method were higher.The specific fragments could be amplified from C.perfringens,but no products were obtained from other Clostridium strains,Escherichia coli and Staphylococcus.The sensitivity of the PCR assay was 1.2× 104 CFU/mL.Forty samples of cattle feces were collected for PCR detecti…查看全部>>
王光华;陆艳;王戈平;蔡其刚;李秀萍;叶成玉;马利青
青海省畜牧兽医科学院,青海西宁810016青海省畜牧兽医科学院,青海西宁810016青海省畜牧兽医科学院,青海西宁810016青海省畜牧兽医科学院,青海西宁810016青海省畜牧兽医科学院,青海西宁810016青海省畜牧兽医科学院,青海西宁810016青海省畜牧兽医科学院,青海西宁810016
农业科技
产气荚膜梭菌毒素多重PCR
Clostridium perfringens toxins multiplex PCR
《畜牧与兽医》 2013 (2)
22-25,4
青海省农牧厅-省校联合项目(QNH-2010-395).
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