中国草食动物科学2013，Vol.33Issue(4)：14-18,5.DOI:10.3969/j.issn.2095-3887.2013.04.004
肌醇类SIB对云南半细毛羊精液冷冻保护效果影响的研究
Effects of SIB Cyclohexanediol on the Cryopreservation of Semen in Yunnan Hemi-fine Wool Sheep
摘要
Abstract
In order to decrease the mechanical injuries caused by ice formation during freeze on ram spermatozoa of Yunnan semi-fine wool sheep,a synthetic ice blocker derived from cyclohexanhexol (1,4-CHD) was firstly used in cryopreservation of ram spermatozoa in this study.Briefly,following collection by electric stimulation,equilibration at 4℃ following dilution with the freezing extender,and pre-freezing in liquid nitrogen vapor,the spermatozoa were preserved in liquid nitrogen.The results indicated that 1,4-CHD could not increase the motility of frozen spermatozoa.With the elevation of the concentration of 1,4-CHD,the motility and moving velocity of frozen spermatozoa decreased steadily.Additionally,1,4-CHD could not increase the intact acrosome and membrane percentage of frozen spermatozoa.When the isotonic binding buffer was used to dilute the thawed spermatozoa,the percentage of cells labeled with propidium iodide (PI) after cryopreservation in the presence of 1,4-CHD was significantly higher than that of spermatozoa frozen in the absence of 1,4-CHD (P＜0.05).However,the percentage of frozen-thawed spermatozoa with exposed PS in the presence of 1,4-CHD was significantly less than that of spermatozoa frozen in the absence of 1,4-CHD (P＜0.01).When the basic extenders with an osmolarity of 404 mOsm,528 mOsm,648 mOsm,or 853 mOsm were used to dilute the frozen-thawed spermatozoa respectively,there were no significant differences among the four groups with respect to the moving velocity and membrane integrity (P＞0.05).In conclusion,1,4-CHD can't improve the motility,moving velocity,acrosome staus and membrane integrity of frozen spermatozoa.However,1,4-CHD may inhibit early apoptosis caused by freezing and thawing.关键词
绵羊精子/冷冻/冰晶抑制剂/环己二醇Key words
ram spermatozoa/cryopreservation/synthetic ice blocker/ cyclohexanediol分类
农业科技引用本文复制引用
权国波,李东江,黄羽,李卫娟,洪琼花..肌醇类SIB对云南半细毛羊精液冷冻保护效果影响的研究[J].中国草食动物科学,2013,33(4):14-18,5.基金项目
国家绒毛用羊产业技术体系(CARS-40) （CARS-40）
家养动物平台(NIAGR) （NIAGR）
