中国草食动物科学2013，Vol.33Issue(4)：47-50,4.DOI:10.3969/j.issn.2095-3887.2013.04.015
兔瘟重组VP60蛋白间接ELISA抗体诊断方法的建立
Development of VP60 ELISA for Detecting Antibody to Rabbit Hemorrhagic Disease Virus
摘要
Abstract
To develop a method for quickly detection of rabbit hemorrhagic disease virus antibody,VP60 gene major antigen region was amplified by RT-PCR technique with specific primers based on published RHDV genome sequence;then the target fragment was directionally cloned into PGEX-4T-1 vector;the recombinant plasmid was transformed into E.coli BL21 (DE3),the recombinant proteins were expressed after induced by IPTG.Using the purified recombinant VP60 protein as coating antigen,an indirect VP60-ELISA was developed to detect antibody to RHDV.The recombinant VP60 protein had a good immunogenicity.The VP60-ELISA,having good repeatability,sensitivity and specificity,could be used as a simple and rapid serology detection method for monitoring of anti-RHDV antibody and epidemiologic survey of RHDV.关键词
兔病毒性出血症病毒/VP60蛋白/截短表达/间接ELISA/诊断Key words
rabbit hemorrhagic disease virus/ VP60 protein/ truncated expression/ indirect E LISA/ diagnosis分类
农业科技引用本文复制引用
祖立闯,王金良,肖跃强,沈志强..兔瘟重组VP60蛋白间接ELISA抗体诊断方法的建立[J].中国草食动物科学,2013,33(4):47-50,4.基金项目
山东省自主创新成果转化重大专项计划(2008ZHZX1A1103) （2008ZHZX1A1103）
