中国普通外科杂志2013，Vol.22Issue(7)：885-889,5.DOI:10.7659/j.issn.1005-6947.2013.07.014
缺氧诱导因子1α在肝癌细胞上皮-间充质化中的作用
Function of hypoxia inducible factor 1α in epithelial-mesenchymal transition of liver cancer cells
摘要
Abstract
Objective:To investigate the role of hypoxia inducible factor 1α (HIF-1α) in epithelial-mesenchymal transition (EMT) process ofhepatocellular carcinoma.Methods:HIF-1α inducible liver cancer cell line HepG2Tet-on-HIF-1α cells were used.First,the mRNA and protein expressions of EMT-related molecules (E-cadherin,vimentin and FSP-1) and HIF-1α in HepG2Tet-on-HIF-1α cells under hypoxia were determined by real-time PCR and Westem blot analysis,respectively.Then,under normoxia condition,the expressions of above molecules were determined in HepG2Tet-on-HIF-1α cells with HIF-1α overexpression induced by doxycycline (Dox),and HepG2Tet-on-HIF-1α cells transfected with HIF-1α siRNA after Dox treatment.Results:After hypoxia treatment,the mRNA and protein expressions of EMT-related molecules and HIF-1α in HepG2Tet-on-HIF-1α cells were all significantly increased compared with normoxia status (all P＜0.05).Under normoxia condition,HIF-1 α was overexpressed,and the EMT-related molecules expressions were simultaneously increased significantly in HepG2Tet-on-HIF-1α by Dox exposure (all P＜0.05),but the above effect of Dox was abolished by HIF-1α siRNA transfection.Conclusion:HIF-1α can promote EMT process in HepG2 cells,and probably may be an effective target for liver cancer gene therapy.关键词
癌,肝细胞/上皮-间充质化/缺氧诱导因子1,α亚基Key words
Carcinoma, Hepatocellular/ Epithelial-Mesenchymal Transition/ Hypoxia-Inducible Factor 1, αSubunit分类
医药卫生引用本文复制引用
金炜东,马丹丹,蔡逊,梅洪亮,黄致远..缺氧诱导因子1α在肝癌细胞上皮-间充质化中的作用[J].中国普通外科杂志,2013,22(7):885-889,5.基金项目
国家自然科学基金资助项目(30371395). （30371395）
