中国兽医科学2013，Vol.43Issue(7)：678-683,6.
Le和Tr序列对新城疫Class Ⅰ毒株复制及转录水平的影响
Effects of Le and Tr sequences of Newcastle disease virus for Class Ⅰ on viral replication and transcription
摘要
Abstract
In order to study the effects on the level of viral transcription and replication of the Leader (Le) and Trailer(Tr) sequences of Newcastle disease virus(NDV),the 9a5b minigenome was used as a platform,according to the Le and Tr sequences,to predict their RNA secondary structure.Through a series of deletion,mutation,replacement,six mutant minigenomes were constructed.Co-transfection,real time quantitative RT-PCR experiments showed that the replacement of the Le end of 9～28 nt by Tr end of 9～28 nt did not affect the efficiency of the viral transcription and replication.Also,direct lack of Le of the second stem-loop structure or of the Le end of the 25 nt did not significantly affect the viral transcription and replication.But when the missing Tr mid-42 nt,or end of 26 nt,the viral replication and transcription terminated.The results prove that the conservation of the Tr sequence is more important than the Le sequence in NDV replication and transcription.关键词
微型基因组/前导序列/尾随序列Key words
minigenome / Leader sequence / Trailer sequence分类
生物科学引用本文复制引用
于洋,何孔旺,封振,仇旭升,孙英杰,丁铲..Le和Tr序列对新城疫Class Ⅰ毒株复制及转录水平的影响[J].中国兽医科学,2013,43(7):678-683,6.基金项目
公益性行业(农业)科研专项(201003012) （农业）
国家高技术研究发展计划(863)项目(2011AA10A209) （863）
