中国兽医科学2013，Vol.43Issue(7)：744-748,5.
抗柱状黄杆菌单克隆抗体杂交瘤细胞株的建立及其免疫学特性鉴定
Establishment of hybridoma cell lines secreting anti-Flavobacterium columnare monoclonal antibody and identification of their immunological properties
摘要
Abstract
To prepare for monoclonal antibody(McAb) against Flavobacterium columnare,BALB/c mice were immunized with a particle antigen.The spleen cells from the immunized mice were fused with mouse myeloma cells SP2/0.Two hybridoma cell lines(2B5 and 4G7) stably secreting McAb against F.columnare were obtained by limited dilutions.The antibody secreted belonged to IgG1 subtype with a κ light chain.No cross-reactions were observed between the McAb prepared and other aquatic pathogenic bacteria such as Edwardsiella tarda,Escherichia coli,Aeromonas hydrophila,Vibrio anguillarum,Vibrio alginolyticus,Vibrio parahaemolyticus and Vibrio harveyi.The method was established successfully in generating McAb against F.columnare for the first time,which provides the foundation for the rapid detection of F.columnare.关键词
柱状黄杆菌/单克隆抗体/杂交瘤细胞/免疫学特性Key words
Flavobacterium columnare / monoclonal antibody (McAb) / hybridoma cells / immunological properties分类
农业科技引用本文复制引用
吕娜,张懋岚,张虹茜,殷晓平,赵国坤,孙强,张捷..抗柱状黄杆菌单克隆抗体杂交瘤细胞株的建立及其免疫学特性鉴定[J].中国兽医科学,2013,43(7):744-748,5.基金项目
浙江省科技厅公益项目(2010C32066) （2010C32066）
