浙江农林大学学报2013，Vol.30Issue(4)：627-632,6.
进口黄檀属木材DNA提取与分子鉴定方法初步研究
DNA extraction and molecular identification of imported Dalbergia wood
摘要
Abstract
Establishing a reliable and cost effective method to identify and distinguish wood is important in preventing the illegal timber trade.To overcome the difficulty of distinguishing Dalbergia,which consists of many valuable species,by morphological methods,molecular genetic tools to control species identity were studied.Seven species of Dalbergia wood,D.melanaoxylon,D.oliveri,D.retusa,D.louvelii,D.greveana,D.cochinchinensis,D.cultrate,imported from different countries and one domestic species D.odorifera were selected for testing.Next,an array of different wood DNA extraction and purification methods,including DNeasy Plant Mini Kit,hexadecyltrimethylammonium bromide (CTAB),sodium dodecyl sulfate (SDS) and Magnetic Beads based method,were tried; For the amplification of DNA from wood samples,two PCR reactions were successively performed (nested PCR) and allowed the amplication of one coding region of chloroplast DNA (cpDNA).Results showed that a CTAB-SDS-Magnetic Beads based method was available for Dalbergia wood DNA extraction.Then,433 bp matK gene fragments were amplified and sequenced from the seven species,of which a total of 12 single nucleotide polymorphisms were found.These polymorphic sites were able to distinguish the eight Dalbergia species.Thus,establishment of this method for DNA isolation and polymerase chain reaction (PCR) amplification makes Dalbergia wood amenable to DNA-based identification.关键词
林业工程/黄檀属木材/DNA提取/纯化/巢式PCR/matK基因/鉴定Key words
forest engineering/ Dalbergia timber/ DNA extraction/ purification/ nested-polymerase chain reaction (PCR) / matK / identification分类
农业科技引用本文复制引用
伏建国,刘金良,杨晓军,安榆林,骆嘉言..进口黄檀属木材DNA提取与分子鉴定方法初步研究[J].浙江农林大学学报,2013,30(4):627-632,6.基金项目
国家质量监督检验检疫总局科技项目(2008IK237) （2008IK237）
"十二五"国家科技支撑计划项目(2012BAK11B03) （2012BAK11B03）
