中药材2013，Vol.36Issue(5)：695-698,4.
正交设计法优选盾叶薯蓣胚性愈伤诱导的实验研究
Study on Inducing Embryogenic Callus of Dioscorea zingiberensis by Orthogonal Design
摘要
Abstract
Objective:To select the suitable medium to induce embryogenic callus of Dioscorea zingiberensis.Methods:Plantlet of Dioscorea zingiberesis in vitro was obtained by using apical meristem as explant.The different parts of the plantlets were cultured to select the best explant used for inducing callus and embryoids.Growing rate and diosgenin content were calculated in orthogonal test to optimize combination of phytohormones for inducing embryogenic callus.Results:The leaves were suitable explants to induce callus and embryoid.The inducing rate of callus and embryoids reached 92.5％ and 42.5％,respectively.The optimal medium for inducing embryogenic callus was MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + 2,4-D 1.0 mg/L.Conclusion:The results of this study can be used for effective induction of embryogenic callus of Dioscorea zingiberensis,and lay the foundation for the subsequent research of artificial seeds.关键词
盾叶薯蓣/胚性愈伤/正交实验Key words
Dioscorea zingiberensis C.H.Wright/ Embryogenic callus/ Orthogonal test分类
医药卫生引用本文复制引用
黄和平,高山林,黄璐琦,黄鹏,汪电雷..正交设计法优选盾叶薯蓣胚性愈伤诱导的实验研究[J].中药材,2013,36(5):695-698,4.基金项目
国家科技重大专项(2009ZX09301-005-03-03) （2009ZX09301-005-03-03）
安徽省高等学校自然科学研究重点项目(KJ2011A191) （KJ2011A191）
