中药材2013，Vol.36Issue(5)：725-731,7.
不同品种不同产地大黄UPLC指纹图谱研究
UPLC Fingerprint of Rhei Radix et Rhizoma from Different Habitats
摘要
Abstract
Objective:To establish the UPLC fingerprint of Rhei Radix et Rhizoma and reference crude drugs,analyze the characteristics among fingerprints of three species of reference crude drugs and the common components of Rhei Radix et Rhizoma,and compare the application of different analysis methods.Methods:UPLC procedure was performed on ACQUITY BEH C18 chromatographic column with mobile phase consisted of water (contained 0.1％ phosphoric acid)-acetonitrile (gradient elution) at a flow rate of 0.21 mL/min.Detection wavelength was set at 260 nm and the column temperature was set at 30 ℃.Fingerprints were analyzed by similarity evaluation,cluster analysis and principal component analysis.Results:There were obvious characteristics among fingerprints of three species of reference crude drugs,19 common chromatographic peaks were obtained from Rhei Radix et Rhizoma and 14 peaks were identified according to standard reference substances and by HPLC-MS.The cluster analysis and similarity evaluation showed the same result that 21 batches of sample were grouped into 5 categories and the result had no direct correlation with the botanical species.Both the contents of 4 important ingredients suggested by principal component analysis and the whole fingerprint analysis were necessary in quality evaluation of Rhei Radix et Rhizoma.There was certain limitation in quality evaluation of multiple sources drug which analysis by similarity evaluation and cluster analysis.Conclusion:The method with good reproducibility and separation saves time and solvent,it can be used in identification of three species of reference crude drugs but can not be used in species identification of commercial Rhei Radix et Rhizoma.关键词
大黄/指纹图谱/相似度分析/聚类分析/主成分分析Key words
Rhei Radix et Rhizoma/ Fingerprint / Similarity analysis / Clustering analysis / Principal component analysis分类
医药卫生引用本文复制引用
杜清涛,温金莲,严优芍,郭丽冰..不同品种不同产地大黄UPLC指纹图谱研究[J].中药材,2013,36(5):725-731,7.基金项目
国家自然科学基金(81073045) （81073045）
