中药材2013，Vol.36Issue(5)：780-783,4.
平性药桃仁对寒热不同血瘀证大鼠cAMP-PKA信号通路的影响
Study on the Regulation Role of Semen Persicae to cAMP-PKA Signal Pathway in the Rats with Cold and Heat Blood Stasis Syndrome
摘要
Abstract
Objective:To study the regulation role of with neutral property to cAMP-PKA pathway in the rats with cold and heat blood stasis syndromes and it's mechanism.Methods:60 rats were randomly divided into normal control group,model control group,Semen Persicae group,radix salvia miltiorrhiza group,rhizoma chuanxiong group,12 rats per group.The three herb groups were orally given relative herbs decoction,whose dosages were equal to 10 times the human clinical dose,normal and model control groups were orally given water,2 times/day,20 mL/kg,for 7 days.Experiments in rats with cold and heat blood stasis syndromes were carried on respectiverly.In heat blood stasis syndromes,except normal control group,the other groups were intraperitoneally injected 10％ carrageenan,5 mL/kg,1 times/day,for 3 days;24 hours after the last injection,subcutaneously injected 20％ dry yeast suspension,10 mL/kg.In cold blood stasis syndromes,except normal control group,the other groups were put into fridge,temperature-(18 ± 2) ℃,2 hours/ times,2 times/day,for 7 days.Separately draw 5ml abdominal aortic blood and taken abdominal aorta,6 hours and 12 hours after finishing the model in the two syndromes.Tested the cAMP content by elisa,tested the PKA protein expression by Western blot.Results:Semen Persicae with neutral property,could decrease the content of cAMP in plasma(P ＜ 0.01),inhibit the expression of protein PKA (P ＜ 0.05) in rats with heat stagnation and blood stasis syndrome,increase the plasma content of cAMP(P ＜ 0.01) and increase the expression of protein PKA (P ＜0.01)in rats with cold stagnation and blood stasis syndrome.Semen Persicae had two-way adjustment action on CAMP-PKA signal pathway.Conclusion:In different internal environment of heat stagnation and blood stasis syndrome,cold stagnation and blood stasis syndrome,Semen Persicae with neutral property has two-way adjustment to cAMP-PKA signaling channel,which may be one of the mechanism of it's two-way application.关键词
平性药/桃仁/信号通路Key words
Herb with neutral property/Semen Persicae/Signal pathway分类
医药卫生引用本文复制引用
郝二伟,邓家刚,杜正彩,包传红,芦晓燕,邓秀琼,唐志玲..平性药桃仁对寒热不同血瘀证大鼠cAMP-PKA信号通路的影响[J].中药材,2013,36(5):780-783,4.基金项目
国家科技部973计划课题(2007CB512602) （2007CB512602）
广西科技基础条件平台建设项目(10-046-04) （10-046-04）
