中药材2013，Vol.36Issue(5)：803-806,4.
湖北麦冬有效部位总皂苷的提取纯化工艺研究
Study on Extraction and Purification Technology of Hubei Ophiopogon Saponins
摘要
Abstract
Objective:To explore the extraction and purification technology of total saponins from the effective parts of Liriope spicata.Methods:Orthogonal design was used.Macroporous resin was selected to separate and purify total saponin from the effective parts of Liriope spicata.The process validation was conducted.The total saponins was determined by Ultraviolet Spectrophotometry.Results:The optimal extraction conditions were as follows:l0 times the amount of ethanol(70％) for each occasion and hot reflux(3 × 2 h).Total saponins was purified by D101 macroporous resin.The concentration of eluting ethanol was 50％ ～70％.Ethanol(70％)was selected as the best eluent.The result of process validation was consistent with the study.Conclusion:The process is simple and stable enough to significantly improve the extraction rate of the effective parts.The study can provide reference for the research and production of effective parts of traditional Chinese medicine such as Liriope spicata.关键词
湖北麦冬/有效部位/纯化工艺Key words
Liriope spicata (Thunb.) Lour.var.prolifera Y.T.Ma/ Effective parts / Purification process分类
医药卫生引用本文复制引用
林韵涵,李崇明,李晓东,向阳,张雅琴,张晓存,刘霞..湖北麦冬有效部位总皂苷的提取纯化工艺研究[J].中药材,2013,36(5):803-806,4.基金项目
中央高校基本科研业务费专项基金资助(2012-Ⅳ-107) （2012-Ⅳ-107）
