安徽农业科学Issue(14)：6276-6279,4.
基于鸭胚原代肝细胞的抗乙肝病毒药物评价模型的建立
Establishment of the Model for Anti-hepatitis B Virus Drug Evaluation in Primary Duck Fetal Hepatocytes
摘要
Abstract
[Objective] To establish the model for anti-hepatitis B virus (HBV) drug evaluation in duck fetal hepatocytes.[Method] The ordinary PCR assay was employed to confirm the embryonated,un-hatched,duck eggs layed by the duck hepatitis B virus (DHBV)-infected ducks.Duck fetal hepatocytes were isolated and primary cultured.The SYBR green dye-based fluorescence quantitative PCR assay was established for detection of DHBV-DNA in primary duck fetal hepatocytes.[Result] Duck fetal hepatocytes were isolated from 18-22-day-old duck embryo and their optimized parameters of isolation and culture were determined.The SYBR green dye-based quantitative PCR assay for detection of DHBVDNA was established in primary duck fetal hepatocytes.[Conclusion] The study established a model to evaluate anti-HBV drug by the SYBR green dye-based fluorescence quantitative PCR assay for detection of DHBV-DNA in primary duck fetal hepatocytes.Furthermore,the model was demonstrated to be a sample,specific,sensitive and repeatable assay for screening and evaluation of anti-HBV drugs.关键词
乙肝模型/鸭乙肝病毒/鸭胚肝细胞/原代培养/荧光定量PCR检测Key words
Hepatitis B model/ Duck hepatitis B virus/ Duck fetal hepatocytes/ Primary culture/ Fluorescence quantitative PCR assay分类
农业科技引用本文复制引用
贾袁媛,沈钰明,伍义行,俞晓平..基于鸭胚原代肝细胞的抗乙肝病毒药物评价模型的建立[J].安徽农业科学,2013,(14):6276-6279,4.基金项目
教育部科技研究重点项目(212073) （212073）
科技援疆计划项目(201191260). （201191260）
